Evidence map›Paper›PMID 42210273›Full record

ArticleBMC veterinary research2026

Establishment of methods for visual and rapid detection of piscine lactococcosis based on isothermal recombinase polymerase amplification.

Hanyu Luo, Zhihao Wu, Weiqi Fan, Hui Zeng, Jie Li, Yong-An Zhang, Yang Zhou

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Article in BMC veterinary research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Hanyu LuoNational Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Hainan Research Institute, College of Biomedicine and Health, College of Fisheries, Huazhong Agricultural University, Wuhan, China.
Zhihao WuNational Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Hainan Research Institute, College of Biomedicine and Health, College of Fisheries, Huazhong Agricultural University, Wuhan, China.
Weiqi FanNational Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Hainan Research Institute, College of Biomedicine and Health, College of Fisheries, Huazhong Agricultural University, Wuhan, China.
Hui ZengNational Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Hainan Research Institute, College of Biomedicine and Health, College of Fisheries, Huazhong Agricultural University, Wuhan, China.
Jie LiNational Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Hainan Research Institute, College of Biomedicine and Health, College of Fisheries, Huazhong Agricultural University, Wuhan, China.
Yong-An ZhangNational Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Hainan Research Institute, College of Biomedicine and Health, College of Fisheries, Huazhong Agricultural University, Wuhan, China. yonganzhang@mail.hzau.edu.cn.
Yang ZhouNational Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Hainan Research Institute, College of Biomedicine and Health, College of Fisheries, Huazhong Agricultural University, Wuhan, China. zhouyang@mail.hzau.edu.cn.

Funding

National Key Research and Development Program of China 2023YFD2400700
6 · The paper itself

Abstract

backgroundLactococcosis is a bacterial disease in fish caused by several Lactococcus species, including L. garvieae and L. petauri, with clinical signs frequently overlapping with those caused by other streptococcal species. This diagnostic overlap necessitates a prompt and precise diagnostic tool for guiding accurate treatment and control. To address this diagnostic challenge, we developed basic recombinase polymerase amplification (basic-RPA) and RPA combined with lateral flow dipstick (RPA-LFD) assays for the detection of lactococcosis-associated Lactococcus species.

resultsBoth methods exhibited a detection limit of 10

conclusionsThe established methods are straightforward, sensitive, and specific, offering a promising candidate tool for the rapid on-site diagnosis of piscine lactococcosis.

Indexed as

Fish DiseasesGram-Positive Bacterial InfectionsLactococcusNucleic Acid Amplification TechniquesAnimalsDNA, BacterialFishesRapid Diagnostic TestsRecombinasesSensitivity and SpecificityDNA, BacterialRecombinasesLactococcosisLactococcus speciesLateral flow dipstick (LFD)Rapid detectionRecombinase polymerase amplification (RPA)

Identifiers

PMID42210273
PMCPMC13418454

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.