Cell-type-specific compartmentalization and function of the glucosinolate-myrosinase system in Arabidopsis thaliana.

Shweta Chhajed, Yatendra Singh, Hajra Maqsood, Craig Dufresne, Wenyuan Song, Sixue Chen

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Article in The Journal of biological chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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6 authors.

Shweta ChhajedDepartment of Biology, University of Florida, Gainesville, Florida, USA.

Yatendra SinghDepartment of Biology, University of Mississippi, Oxford, Mississippi, USA.

Hajra MaqsoodDepartment of Biology, University of Mississippi, Oxford, Mississippi, USA.

Craig DufresneDivision of Chromatography, Thermo Scientific Training Institute, West Palm Beach, Florida, USA.

Wenyuan SongDepartment of Plant Pathology, IFAS, University of Florida, Gainesville, Florida, USA.

Sixue ChenDepartment of Biology, University of Florida, Gainesville, Florida, USA; Department of Biology, University of Mississippi, Oxford, Mississippi, USA; Plant Molecular and Cellular Biology, University of Florida, Gainesville, Florida, USA; Genetics Institute, University of Florida, Gainesville, Florida, USA. Electronic address: schen8@olemiss.edu.

Funding

NSF 2340995USDA 1024092

6 · The paper itself

Abstract

Plants deploy chemical defense strategies to respond to abiotic and biotic stresses, including the glucosinolate-myrosinase (GM) system ("mustard oil bomb") characteristic of Brassicales. Glucosinolates (GLSs) are specialized metabolites that, upon hydrolysis by myrosinases, generate bioactive products that deter herbivores and pathogens. While the biochemical functions of GLS breakdown products are well studied, the cell-type-specific organization of the GM system and its relevance to stomatal immunity remain incompletely defined. Here, we compare cell-type-enriched GLS profiles and myrosinase activities in guard cells (GCs) and mesophyll cells (MCs) of Arabidopsis thaliana. Using untargeted metabolomics, we identified 20 GLSs across seeds, leaves, GCs, and MCs with high mass accuracy, followed by targeted quantitative analysis of GLSs based on a selected reaction monitoring approach. Comparative analyses revealed pronounced differences in GLS composition between GCs and MCs, accompanied by distinct myrosinase activity profiles. Together, these findings establish a cell-type-specific model for GLS metabolism in A. thaliana and demonstrate differential organization of the GM system between GCs and MCs. This work provides foundational evidence linking the spatial organization of GLS metabolism to stomatal immune function and will springboard future mechanistic studies of the GM system in plant defense signaling.

Indexed as

ArabidopsisArabidopsis ProteinsGlucosinolatesGlycoside HydrolasesArabidopsis ProteinsGlucosinolatesGlycoside HydrolasesthioglucosidaseArabidopsis thalianaglucosinolate-myrosinase systemglucosinolatesguard and mesophyll cellsselected reaction monitoring

Identifiers

PMID42208903

DOI10.1016/j.jbc.2026.113202

PMCPMC13315440

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