Therapeutic effect of

Background: Methods: This study investigated the therapeutic effects and underlying mechanisms of THH in a murine model by employing 16S rRNA gene sequencing, intestinal metabolomic profiling, fecal microbiota transplantation (FMT), and co-housing experiments. Results: Our results demonstrated that THH treatment significantly decreased PASI scores, alleviated epidermal hyperplasia and skin inflammation, and reversed IMQ-induced gut dysbiosis by restoring the Firmicutes/Bacteroidota ratio and modulating the abundance of beneficial and pathogenic bacteria. Metabolomic analysis revealed that THH normalized multiple metabolic pathways disturbed by IMQ, including arachidonic acid metabolism, sphingolipid metabolism, and primary bile acid biosynthesis. Correlation analyses further revealed significant associations among the altered gut microbiota, key metabolic pathways, and psoriasis-related phenotypic indices. Moreover, FMT from THH-treated mice conferred significant anti-psoriatic efficacy. Similarly, co-housing experiments resulted in the alleviation of skin lesions, reduction of spleen weight, and downregulation of inflammatory cytokines. Conclusion: These findings demonstrate that THH attenuates psoriasis-like dermatitis partly by reshaping gut microbiota composition and regulating key metabolic pathways, supporting a potential gut-targeted therapeutic strategy for psoriasis.