Evidence map›Paper›PMID 42205231›Full record

ArticleBiotechnology reports (Amsterdam, Netherlands)2026

Overcoming challenges in the production of a human-like L-asparaginase: advanced strategies for expression and purification.

Maísa Pessoa Pinheiro, Ana Virgínia Frota Guimarães, Lucas Almeida de Freitas, Gilvan Pessoa Furtado, Marcos Roberto Lourenzoni, Marco Alberto Medeiros

Abstract read
In one paragraph

Article in Biotechnology reports (Amsterdam, Netherlands), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Maísa Pessoa PinheiroFundação Oswaldo Cruz (Fiocruz), Protein Engineering and Healthcare Solution Group (GEPeSS), Fortaleza, CE 61760-000, Brazil.
Ana Virgínia Frota GuimarãesFundação Oswaldo Cruz (Fiocruz), Protein Engineering and Healthcare Solution Group (GEPeSS), Fortaleza, CE 61760-000, Brazil.
Lucas Almeida de FreitasFundação Oswaldo Cruz (Fiocruz), Protein Engineering and Healthcare Solution Group (GEPeSS), Fortaleza, CE 61760-000, Brazil.
Gilvan Pessoa FurtadoFundação Oswaldo Cruz (Fiocruz), Protein Engineering and Healthcare Solution Group (GEPeSS), Fortaleza, CE 61760-000, Brazil.
Marcos Roberto LourenzoniFundação Oswaldo Cruz (Fiocruz), Protein Engineering and Healthcare Solution Group (GEPeSS), Fortaleza, CE 61760-000, Brazil.
Marco Alberto MedeirosFundação Oswaldo Cruz (Fiocruz), Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos), Rio de Janeiro, RJ 21040-360, Brazil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Human l-asparaginase 1 (hASNase1) is a promising next-generation candidate for Acute Lymphoblastic Leukemia (ALL) therapy due to its reduced immunogenicity and superior physiological compatibility. However, its recombinant production remains challenging, as expression in

Indexed as

Acute lymphoblastic leukemiaHuman L-asparaginase 1Mild expression conditionsProtein aggregationSolubility

Identifiers

PMID42205231
PMCPMC13202022

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.