Evidence map›Paper›PMID 42204619›Full record

ArticleGenome biology2026

Real-time search-assisted multiplexed quantitative proteomics reveals system-wide cryptic translation initiation in human cancer cells.

Hiroko Kozuka-Hata, Tomoko Hiroki, Aya Kitamura, Naoaki Miyamura, Tetsu Akiyama, Jun-Ichiro Inoue, Kouhei Tsumoto, Masaaki Oyama

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Article in Genome biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

Who cites it

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Hiroko Kozuka-HataMedical Proteomics Laboratory, The Institute of Medical Science, The University of Tokyo, 4-6-1, Shirokanedai, Minato-Ku, Tokyo, 108-8639, Japan.
Tomoko HirokiMedical Proteomics Laboratory, The Institute of Medical Science, The University of Tokyo, 4-6-1, Shirokanedai, Minato-Ku, Tokyo, 108-8639, Japan.
Aya KitamuraMedical Proteomics Laboratory, The Institute of Medical Science, The University of Tokyo, 4-6-1, Shirokanedai, Minato-Ku, Tokyo, 108-8639, Japan.
Naoaki MiyamuraMedical Proteomics Laboratory, The Institute of Medical Science, The University of Tokyo, 4-6-1, Shirokanedai, Minato-Ku, Tokyo, 108-8639, Japan.
Tetsu AkiyamaLaboratory of Molecular and Genetic Information, Institute for Quantitative Biosciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-Ku, Tokyo, 113-0032, Japan.
Jun-Ichiro InoueThe University of Tokyo Pandemic Preparedness, Infection and Advanced Research Center (UTOPIA), 4-6-1, Shirokanedai, Minato-Ku, Tokyo, 108-8639, Japan.
Kouhei TsumotoMedical Proteomics Laboratory, The Institute of Medical Science, The University of Tokyo, 4-6-1, Shirokanedai, Minato-Ku, Tokyo, 108-8639, Japan.
Masaaki OyamaMedical Proteomics Laboratory, The Institute of Medical Science, The University of Tokyo, 4-6-1, Shirokanedai, Minato-Ku, Tokyo, 108-8639, Japan. moyama@ims.u-tokyo.ac.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundIt is generally considered that eukaryotic translation initiation prominently occurs from the first AUG codon by ribosomal scanning from the 5-cap end of each mRNA. In order to identify cryptic internal translation initiation sites defined by alternative AUG codons on a proteome-wide scale, we generate a customized amino acid sequence database which contain differential AUG-guided tryptic peptide fragments computationally predicted from well-curated Swiss-Prot human protein reference sequences and applied it for high-resolution mass spectrometry-based proteomic analysis.

resultsThe ultra-deep proteomic detection based on the real-time search platform on Orbitrap Eclipse Tribrid mass spectrometry system leads to identification of not only more than 26,000 unique peptides from already annotated human protein coding sequences but also 794 novel peptide fragments defined by alternative downstream translation initiation in human cancer cells. Very notably, Tandem Mass Tag-based multiplex quantitative analysis of patient-derived glioblastoma initiating cells uncovers epidermal growth factor-dependent translational regulation on a wide range of differential AUG-guided non-canonical proteoforms as well as cancer-related transcription factors and cell cycle/cell division regulators in a cell-type specific manner.

conclusionsOur study provides the first proteome-wide evidence of downstream AUG-guided cryptic translation initiation dynamics in human cancer cells.

Indexed as

Peptide Chain Initiation, TranslationalProteomicsCell Line, TumorCodon, InitiatorHumansProteomeCodon, InitiatorProteome

Identifiers

PMID42204619
PMCPMC13404309

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.