ArticleAnalytical and bioanalytical chemistry2026
Derivatisation-free FIA-MS/MS assay for rapid simultaneous screening of atypical myopathy biomarkers and acylcarnitine profiles from dried blood spots.
Article in Analytical and bioanalytical chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Atypical myopathy (AM) is a frequently fatal, toxin-induced disease in horses, characterised by acute progression and mortality rates exceeding 70% within 72 h. The disease is caused by ingestion of seedlings or seeds of certain maple species that contain the toxins hypoglycin A (HGA) and methylenecyclopropylglycine (MCPrG). Early diagnosis depends on reliable detection of these compounds and their metabolites. However, most available LC-MS/MS assays are limited by derivatisation requirements and dependence on serum and urine matrices. Here, a rapid derivatisation-free flow injection analysis (FIA)-tandem mass spectrometry (MS/MS) method was developed for the simultaneous quantification of nine analytes: HGA, methylenecyclopropylacetyl-carnitine (MCPA-carnitine), and seven diagnostically relevant acylcarnitines, from dried blood spots (DBS). The procedure requires only 50 µL of whole blood, minimal sample preparation, and enables results within one minute. The assay provides low limits of quantification (0.01-0.1 μmol/L) with excellent linearity (R
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