Efficient clarification of apple juice using immobilized pectinase on glass surface in a novel support-free plate bioreactor.

Hamid Mahmood-Fashandi, Faramarz Khodaiyan, Seyed Saeid Hosseini

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Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

3 authors.

Hamid Mahmood-FashandiDepartment of Food Science and Engineering, University of Tehran, Karaj, 31587-77871, Iran.

Faramarz KhodaiyanDepartment of Bioengineering, School of Life Sciences Engineering, College of Interdisciplinary Science and Technologies, University of Tehran, Tehran, 14399-57131, Iran. khodaiyan@ut.ac.ir.

Seyed Saeid HosseiniDepartment of Bioengineering, School of Life Sciences Engineering, College of Interdisciplinary Science and Technologies, University of Tehran, Tehran, 14399-57131, Iran.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study presents the development and evaluation of a novel support-free plate bioreactor (SFPB) for immobilization of pectinase and its application in apple juice clarification. The SFPB was developed as an alternative to the conventional packed-bed bioreactor to address operational limitations when comparing at an equal surface area. Pectinase was covalently immobilized on the inner glass surface using 3-aminopropyl triethoxysilane and glutaraldehyde. The immobilization efficiency in the SFPB was 2.38 times higher than in the PBB. The immobilized enzyme retained approximately 70% of its initial activity after 225 min, compared to 47% in the PBB. Kinetic analysis showed increased Km and decreased Vmax for the immobilized enzyme, indicating reduced substrate affinity and catalytic activity. Under optimal conditions (pH 5.5, 50 °C, 0.8 mL min

Indexed as

BioreactorsEnzymes, ImmobilizedFruit and Vegetable JuicesMalusPolygalacturonaseGlassGlutaralHydrogen-Ion ConcentrationKineticsPropylaminesSilanesTemperatureEnzymes, ImmobilizedGlutaralPolygalacturonasePropylaminesSilanesApple juiceEnzyme immobilizationPacked bed bioreactorPectinaseSupport-free plate bioreactor

Identifiers

PMID42203815

DOI10.1038/s41598-026-54234-4

PMCPMC13443213

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LicenceCC BY-NC-ND

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.