Evidence map›Paper›PMID 42203769›Full record

ArticleNature communications2026

RNAs anchoring replication complex control initiation and firing of DNA replication.

Simone Ummarino, Larysa Poluben, Alexander K Ebralidze, Ida Autiero, Lucrezia Rinaldi, Theodore Paniza, Madhura Deshpande, Nicholas H Mandel, Jonathan D Lee, Yanzhou Zhang and 9 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

19 authors.

Simone Ummarino *Harvard Medical School Initiative for RNA Medicine, Harvard Medical School, Boston, MA, USA.
Larysa Poluben *Harvard Medical School Initiative for RNA Medicine, Harvard Medical School, Boston, MA, USA.
Alexander K Ebralidze *Harvard Medical School Initiative for RNA Medicine, Harvard Medical School, Boston, MA, USA.
Ida AutieroInstitute of Biostructures and Bioimaging, CNR, Naples, Italy.ORCID http://orcid.org/0000-0001-8886-8063
Lucrezia RinaldiHarvard Medical School Initiative for RNA Medicine, Harvard Medical School, Boston, MA, USA.
Theodore PanizaCenter for Reproductive Medicine, Weill Cornell Medical College, New York, NY, USA.
Madhura DeshpandeCenter for Reproductive Medicine, Weill Cornell Medical College, New York, NY, USA.ORCID http://orcid.org/0000-0002-9942-4932
Nicholas H MandelDepartment of Biology, Tufts University, Medford, MA, USA.
Jonathan D LeeHarvard Medical School Initiative for RNA Medicine, Harvard Medical School, Boston, MA, USA.ORCID http://orcid.org/0000-0002-1229-0098
Yanzhou ZhangHarvard Medical School Initiative for RNA Medicine, Harvard Medical School, Boston, MA, USA.
Mahmoud A BassalHarvard Medical School Initiative for RNA Medicine, Harvard Medical School, Boston, MA, USA.
Bogdan BudnikWyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA.ORCID http://orcid.org/0000-0003-3622-2003
Bon Q TrinhDepartment of Pathology, University of Virginia School of Medicine, Charlottesville, VA, USA.
Steven P BalkHarvard Medical School Initiative for RNA Medicine, Harvard Medical School, Boston, MA, USA.ORCID http://orcid.org/0000-0002-4546-7371
Robert FlaumenhaftBeth Israel Deaconess Medical Center, Division of Hemostasis and Thrombosis, Harvard Medical School, Boston, MA, USA.
Jeannine GerhardtCenter for Reproductive Medicine, Weill Cornell Medical College, New York, NY, USA.
Sergei M MirkinDepartment of Biology, Tufts University, Medford, MA, USA. sergei.mirkin@tufts.edu.ORCID http://orcid.org/0000-0003-4576-7582
Daniel G TenenHarvard Medical School Initiative for RNA Medicine, Harvard Medical School, Boston, MA, USA. dtenen@bidmc.harvard.edu.ORCID http://orcid.org/0000-0002-6423-3888
Annalisa Di RuscioHarvard Medical School Initiative for RNA Medicine, Harvard Medical School, Boston, MA, USA. adirusci@bidmc.harvard.edu.ORCID http://orcid.org/0000-0002-9705-4245

Funding

Determining the role of DNMT-interacting RNAs in myeloid disordersR00CA188595 · NCI · BETH ISRAEL DEACONESS MEDICAL CENTER · PI DI RUSCIO, ANNALISA · 2017 to 2019
$747k
NCI NIH HHS R00 CA188595U.S. Department of Health & Human Services | NIH | National Cancer Institute (NCI) R00CA188595
6 · The paper itself

Abstract

Coordinated initiation of DNA replication is essential to ensure efficient and timely DNA synthesis. Yet the molecular determinants that confer origin selectivity in mammalian cells remain incompletely defined. Herein, we present data demonstrating a pivotal role for RNAs transcribed in the proximity of actively replicating gene loci. We show that RNAs aNChoring ORC1 (ANCORs) to the histone variant H2A.Z facilitate origin firing during DNA replication. This ANCOR-H2A.Z interaction appears to be essential for cells to duplicate their genetic material. Widespread and locus-specific perturbations of these transcripts correlate with anomalous replication patterns and a notable loss of the H2A.Z replicative marker at the origin site. Collectively, we present a previously undescribed RNA-mediated mechanism that is associated with the generation of active replication origins in mammalian cells. Our findings delineate a strategy to modulate the origins of replication in human cells at a local and global level, with potentially broad biomedical implications.

Indexed as

DNA ReplicationHistonesOrigin Recognition ComplexRNAAnimalsHeLa CellsHumansReplication OriginHistonesORC1 protein, humanOrigin Recognition ComplexRNA

Identifiers

PMID42203769
PMCPMC13500710

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.