Effect of pulsed ultrasound pretreatment on enzymatic hydrolysis and bio-functional properties of luffa cylindrica seed protein hydrolysates.

Hoda Shahiri Tabarestani, Mohammad Mansour Samie, Alireza Sadeghi Mahoonak, Ali Moayedi

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Article in Ultrasonics sonochemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 authors.

Hoda Shahiri TabarestaniDepartment of Food Science and Technology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. Electronic address: hoda.shahiri@gau.ac.ir.

Mohammad Mansour SamieDepartment of Food Science and Technology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

Alireza Sadeghi MahoonakDepartment of Food Science and Technology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

Ali MoayediDepartment of Food Science and Technology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Pulsed ultrasound pretreatment was investigated as a strategy to enhance the enzymatic hydrolysis and bio-functional properties of Luffa cylindrica seed protein concentrate. The influence of ultrasound power (100-200 W) and treatment time (5-25 min) on the degree of hydrolysis (DH), structural characteristics, functional properties, and antioxidant activity of Alcalase-generated hydrolysates was evaluated. Ultrasound pretreatment resulted in higher DH values compared with conventional enzymatic hydrolysis (8.13% after 4 h). Ultrasound-assisted treatments followed by only 2 h of hydrolysis produced DH values of 9.6-35.3%, with the maximum obtained at 200 W for 25 min, suggesting that ultrasonic pretreatment may accelerate proteolysis while reducing processing time. SDS-PAGE indicated progressive degradation of native proteins, including reduced intensity of high-molecular-weight bands (>45 kDa) and the appearance of peptides below 15 kDa. FTIR analysis suggested ultrasound-induced conformational changes that may facilitate enzyme accessibility. Moderate treatment (200 W, 5 min) was associated with the highest emulsifying activity (33.3 m

Indexed as

LuffaPlant ProteinsProtein HydrolysatesSeedsSubtilisinsUltrasonic WavesAntioxidantsHydrolysisAntioxidantsPlant ProteinsProtein HydrolysatesSubtilisinsAntioxidant activityEmulsifying activityEnzymatic hydrolysisFTIRLuffa cylindricaUltrasound pretreatment

Identifiers

PMID42202417

DOI10.1016/j.ultsonch.2026.107902

PMCPMC13234717

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.