ArticlePlanta2026
RUBY-BD: a highly efficient visual reporter system for assessing bidirectional promoter activity.
Article in Planta, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
MAIN
conclusionWe developed a universal, one-step reporter system, RUBY-BD, enabling high-throughput examination of transcriptional activity of bidirectional promoters. Bidirectional promoters (BDPs), the intergenic regions between divergent gene pairs, enable simultaneous transcription of both genes and are highly valuable for multigene engineering. Conventional reporter systems like green fluorescent protein (GFP) and β-glucuronidase (GUS) are widely used to assess BDP activity; however, their reliance on cumbersome detection methods and high experimental costs hinders high-throughput screening. The RUBY reporter system offers an efficient alternative, requiring only the co-expression of three enzymes (AD1, DODA, GT) to convert tyrosine into betalain, a red-violet pigment visible to the naked eye. In this study, we separated the three genes and the intervening 2A sequence of the RUBY system into two bidirectional transcription units, constructing an RUBY-BD vector. Transient transformation assays in Nicotiana benthamiana leaves confirmed that inserting an active BDP leads to the expression of all three genes and results in betalain biosynthesis. Using the RUBY-BD system, we evaluated the activity of 20 putative plant BDPs in a single step. Betalain accumulation and expression analysis of the two flanking genes confirmed the bidirectional transcriptional activity of 11 BDPs. In summary, this study established a high-throughput visual screening tool for characterizing plant BDPs, which is expected to accelerate basic research into BDPs and their application in gene engineering.
Indexed as
Identifiers
42201409What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.