Evidence map›Paper›PMID 42199473›Full record

ArticleBio-protocol2026

3D Reconstruction of Mature

Takao Oi, Tomoyo Morikawa, Yuna Yamazaki, Daisuke Maruyama, Daichi Susaki

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Takao OiGraduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan.
Tomoyo MorikawaHigh Voltage Electron Microscope Laboratory, Institute of Materials and Systems for Sustainability, Nagoya University, Nagoya, Japan.
Yuna YamazakiDepartment of Biological Science, Faculty of Science, Shizuoka University, Shizuoka, Japan.
Daisuke MaruyamaKihara Institute for Biological Research, Yokohama City University, Yokohama, Japan.
Daichi SusakiDepartment of Biological Science, Faculty of Science, Shizuoka University, Shizuoka, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Volume electron microscopy based on serial sectioning allows for three-dimensional (3D) visualization and analysis of the internal structures of tissues, cells, and organelles. One such technique, focused ion beam (FIB) scanning electron microscopy (SEM), has the advantages of nanoscale sectioning and high z-resolution, but the disadvantage of limited volume processing. Because of this limitation, targeting localized objects by FIB-SEM is difficult. Here, we developed a FIB-SEM observation workflow that enables the analysis of the filiform apparatus of synergid cells enclosed in the

Indexed as

Carbon coatingCell wallChemical fixationElectron microscopyEn bloc stainingFocused ion beamPlantUltramicrotomy

Identifiers

PMID42199473
PMCPMC13200071

What OpenQuestion holds

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LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.