ArticleRegenerative therapy2026
Spontaneously formed mesenchymal stem cell spheroids enhance alveolar bone regeneration and suppress inflammation following tooth autotransplantation.
Article in Regenerative therapy, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Sequential Double Autotransplantation Using Immature Teeth as First-Stage Autotransplants to Preserve Alveolar Architecture: A Case Series.Case reports in dentistry · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Objectives: This study aimed to evaluate the effects of spontaneously formed mesenchymal stromal cells (MSCs) spheroids on alveolar bone regeneration and inflammatory responses following tooth autotransplantation in a murine model. Methods: Spontaneous spheroids were generated from mouse calvaria-derived MC3T3-E1 cells using a low-adhesion culture system. Extracted molars were transplanted with spheroids embedded in a collagen-based scaffold. Monolayer cells, scaffold, and tooth-only groups served as controls. Bone regeneration was assessed by micro-CT and histological analyses at 1, 2, and 4 weeks post-transplantation. Gene expression related to osteogenesis and inflammation was analyzed by quantitative PCR. Results: In vitro analyses demonstrated that MC3T3-E1 spheroids exhibited significantly increased alkaline phosphatase activity, mineralization, and expression of pluripotency and osteogenesis related genes compared with monolayer cells. In vivo, the spheroid group showed significantly enhanced new bone formation, with higher bone volume fraction (BV/TV) throughout the observation period (p < 0.05). Expression of osteogenic markers, including osteocalcin (OCN) and collagen type I, was significantly upregulated, whereas inflammatory cytokines IL-6 and TNF-α were consistently suppressed. Histological evaluation revealed organized collagen fiber alignment suggestive of periodontal ligament-like tissue regeneration. Conclusions: MSC-derived spontaneous spheroids promote alveolar bone regeneration and suppress inflammatory responses following tooth autotransplantation. This spheroid-based strategy may improve regenerative outcomes in tooth autotransplantation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.