Evidence map›Paper›PMID 42198583›Full record

ArticlePathogens (Basel, Switzerland)2026

A Multiplex One-Tube Nested Real-Time PCR Assay for the Point-of-Care Testing of Infectious Meningitis.

Duoxiao Zhang, Jie Wang, Zijin Zhao, Yanqing Tie, Jianing Wu, Shihao Jiao, Xingyu Liu, Yuxin Wang, Shijue Gao, Mengchuan Zhao and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Pathogens (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Duoxiao ZhangGraduate School, Hebei North University, Zhangjiakou 075000, China.ORCID 0009-0000-7014-0277
Jie WangDepartment of Clinical Laboratory, Hebei General Hospital, Shijiazhuang 050051, China.
Zijin ZhaoDepartment of Clinical Laboratory, Hebei General Hospital, Shijiazhuang 050051, China.
Yanqing TieDepartment of Clinical Laboratory, Hebei General Hospital, Shijiazhuang 050051, China.
Jianing WuGraduate School, Hebei North University, Zhangjiakou 075000, China.
Shihao JiaoDepartment of Clinical Laboratory, Hebei General Hospital, Shijiazhuang 050051, China.
Xingyu LiuDepartment of Clinical Laboratory, Hebei General Hospital, Shijiazhuang 050051, China.
Yuxin WangDepartment of Clinical Laboratory, Hebei General Hospital, Shijiazhuang 050051, China.
Shijue GaoNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Mengchuan ZhaoDepartment of Clinical Laboratory, Hebei General Hospital, Shijiazhuang 050051, China.
Pei ZhaoDepartment of Clinical Laboratory, Hebei General Hospital, Shijiazhuang 050051, China.ORCID 0009-0005-4660-4875
Zhiqiang HanDepartment of Clinical Laboratory, Hebei General Hospital, Shijiazhuang 050051, China.
Xiaona LyuDepartment of Clinical Laboratory, Hebei General Hospital, Shijiazhuang 050051, China.
Xinxin ShenNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Xuejun MaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.ORCID 0000-0001-5891-5260
Zhishan FengDepartment of Clinical Laboratory, Hebei General Hospital, Shijiazhuang 050051, China.

Funding

China International Medical Foundation YXTL-2024-0002-0600Government funded clinical medical talents training project ZF2024001Hebei Province Medical Science Research Project 2024021National Natural Science Foundation of China 82202593
6 · The paper itself

Abstract

In this study, we developed a multiplex one-tube nested real-time fluorescent quantitative PCR (mONRT-PCR) assay integrated with a portable, fully automated nucleic acid point-of-care testing (POCT) platform for the detection of

Indexed as

Multiplex Polymerase Chain ReactionPoint-of-Care TestingReal-Time Polymerase Chain ReactionCryptococcus neoformansHaemophilus influenzaeHumansListeria monocytogenesRapid Diagnostic TestsSensitivity and Specificitycerebrospinal fluidCryptococcus neoformansHaemophilus influenzaeListeria monocytogenesmultiplex one-tube nested real-time fluorescent quantitative PCR (mONRT-PCR)multiplex testingPOCT

Identifiers

PMID42198583
PMCPMC13209304

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.