ArticlePolymers2026
Enzyme Immobilisation on Cellulose via Bifunctional Reactive Dyes: A Simple Route to Textile-Based Biocatalysts.
Article in Polymers, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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5 authors.
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Abstract
The durable enzyme functionalisation of cellulosic fibres is often limited by enzyme deactivation under alkaline processing and insufficient wash resistance. Here, a cold pad-batch (CPB)-compatible route integrates reactive dyeing and lysozyme anchoring by using the commercial bifunctional dye C.I. Reactive Red 195 (MCT/VS) as an interfacial mediator to build a cellulose-dye-lysozyme ternary layer. The dye is first fixed on cotton, and residual electrophilic motifs are proposed to facilitate subsequent coupling with nucleophilic residues in lysozyme. A nine-run, four-factor/three-level orthogonal design was used to identify a practical processing window; under the selected condition, an ELISA-equivalent releasable lysozyme level of 53.8 U/L was achieved together with moderate colour strength (K/S = 6.5). The treated fabrics exhibited 96.5% inhibition against
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