Evidence map›Paper›PMID 42197428›Full record

ArticleMicroorganisms2026

Validation of a Pseudovirus Neutralization Assay for Severe Acute Respiratory Syndrome Coronavirus 2 Omicron JN.1 and LP.8.1 Subvariant Lineage Strains with Homologous and Heterologous Matched Sera in Clinically Relevant Samples.

Zhaohui Cai, Raj Kalkeri, Benjamin Haner, Mi Wang, Paul Skonieczny, Bahar Osman, Dominic Dent, David Silva, Kevin Auerbach, Emmanuel Faust and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microorganisms, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Zhaohui CaiClinical Immunology, Novavax, Gaithersburg, MD 20878, USA.
Raj KalkeriClinical Immunology, Novavax, Gaithersburg, MD 20878, USA.
Benjamin HanerClinical Immunology, Novavax, Gaithersburg, MD 20878, USA.
Mi WangClinical Immunology, Novavax, Gaithersburg, MD 20878, USA.
Paul SkoniecznyClinical Immunology, Novavax, Gaithersburg, MD 20878, USA.
Bahar OsmanClinical Immunology, Novavax, Gaithersburg, MD 20878, USA.
Dominic DentClinical Immunology, Novavax, Gaithersburg, MD 20878, USA.
David SilvaClinical Immunology, Novavax, Gaithersburg, MD 20878, USA.
Kevin AuerbachClinical Immunology, Novavax, Gaithersburg, MD 20878, USA.
Emmanuel FaustClinical Immunology, Novavax, Gaithersburg, MD 20878, USA.
Sheau-Line FengClinical Immunology, Novavax, Gaithersburg, MD 20878, USA.
Miranda R CaiClinical Immunology, Novavax, Gaithersburg, MD 20878, USA.
Mingzhu ZhuClinical Immunology, Novavax, Gaithersburg, MD 20878, USA.ORCID 0009-0005-1628-7442
Shane Cloney-ClarkClinical Immunology, Novavax, Gaithersburg, MD 20878, USA.
Joyce S PlestedClinical Immunology, Novavax, Gaithersburg, MD 20878, USA.

Funding

Novavax (United States) N/A
6 · The paper itself

Abstract

The pseudovirus neutralization (PNT) assay is an established high-throughput, robust, and efficient BSL-2 method for detecting neutralizing antibodies (NAbs) against SARS-CoV-2 with the correlates of protection previously established for the ancestral (Wuhan) strain. The PNT assay was validated using nonmatched ancestral sera with anti-JN.1 cross-NAbs, clinically matched JN.1 sera with anti-JN.1 NAbs, or nonmatched JN.1 sera with anti-LP.8.1, KP.2, or KP.3 cross-reacting NAbs. In line with predefined validation acceptance criteria, the PNT assay was precise, with %GCV ≤ 50 in ~90-100%/200 results (40 samples/strain). The acceptance criteria were met for linearity (slope ranged from 1.041 for ancestral sera with anti-JN.1 NAbs to 1.213 for JN.1 sera with anti-KP.2 NAbs),

Indexed as

assay validationcorrelate of protectionCOVID-19immunogenicityOmicron JN.1SARS-CoV-2

Identifiers

PMID42197428
PMCPMC13209548

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.