High-Throughput LC-MS/MS Quantification of Eighteen Cannabinoids in Hemp Flowers with Baseline Separation of Structural Isomers.

Na Liu, Maggie Schoener, Naima Jannath Rimi, Md Imon Hossain, Supraja Regunathan, Robert Powers, Liguo Song

Abstract read

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Article in Molecules (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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7 authors.

Na LiuDepartment of Physical and Biological Sciences, Western New England University, Springfield, MA 01119, USA.ORCID 0009-0007-0194-0757

Maggie SchoenerDepartment of Chemistry, Western Illinois University, Macomb, IL 61455, USA.ORCID 0009-0002-0255-6646

Naima Jannath RimiDepartment of Chemistry, Western Illinois University, Macomb, IL 61455, USA.ORCID 0009-0005-0807-6119

Md Imon HossainDepartment of Chemistry, Western Illinois University, Macomb, IL 61455, USA.ORCID 0009-0004-3274-9910

Supraja RegunathanDepartment of Forensic Science, University of New Haven, West Haven, CT 06516, USA.

Robert PowersDepartment of Forensic Science, University of New Haven, West Haven, CT 06516, USA.

Liguo SongDepartment of Chemistry, Western Illinois University, Macomb, IL 61455, USA.ORCID 0000-0002-8147-3381

Funding

National Institute of Justice 15PNIJ-23-GG-04234-RESSNational Institute of Justice 2020-DQ-BX-0021

6 · The paper itself

Abstract

Following the passage of the Agriculture Improvement Act of 2018, demand for accurate cannabinoid quantification in hemp flowers has increased to ensure regulatory compliance. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) using a triple-quadrupole mass spectrometer provides high sensitivity and selectivity and is well suited for this purpose; however, a review of the literature indicates that many published LC-MS/MS methods target only a limited number of cannabinoids, and reliable differentiation of structural isomers remains challenging. In this study, an LC-MS/MS method was developed for the simultaneous quantification of eighteen cannabinoids in hemp flowers. Baseline chromatographic separation of structural isomers enabled reliable differentiation of compounds with highly similar fragmentation patterns and allowed the use of the most sensitive multiple reaction monitoring (MRM) transitions for quantification. Both positive and negative ionization modes were employed to achieve optimal sensitivity using dynamic polarity switching within a single analytical run. Following validation in accordance with ISO/IEC 17025, the method was applied to a proficiency test hemp sample and six commercial hemp samples, demonstrating excellent time efficiency (11 min for 18 cannabinoids) and an exceptionally wide calibration range (8-5000 ng/mL, corresponding to 0.032-20% (

Indexed as

CannabinoidsCannabisFlowersLiquid Chromatography-Mass SpectrometryTandem Mass SpectrometryChromatography, LiquidHigh-Throughput Screening AssaysIsomerismCannabinoidscannabinoidshemp flowersliquid chromatographyMRMquantificationtriple-quadrupole mass spectrometer

Identifiers

PMID42197238

DOI10.3390/molecules31101684

PMCPMC13209672

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.