Site-Directed Immobilization of DuraPETase onto PET-Binding PDA@SiO

Zixuan Li, Fengyuan Zhang, Shaolei Zhao, Mingbo Sun, Jingru Liu, Yan Xie, Shucai Zhang

Abstract read

In one paragraph

Article in Molecules (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

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2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Zixuan LiState Key Laboratory of Chemical Safety, Qingdao 266104, China.

Fengyuan ZhangState Key Laboratory of Chemical Safety, Qingdao 266104, China.ORCID 0000-0003-2006-0193

Shaolei ZhaoState Key Laboratory of Chemical Safety, Qingdao 266104, China.

Mingbo SunState Key Laboratory of Chemical Safety, Qingdao 266104, China.

Jingru LiuState Key Laboratory of Chemical Safety, Qingdao 266104, China.

Yan XieState Key Laboratory of Chemical Safety, Qingdao 266104, China.

Shucai ZhangState Key Laboratory of Chemical Safety, Qingdao 266104, China.ORCID 0000-0001-5622-6517

Funding

National Key Research and Development Program of China 2024YFC3713800Technology Development Program of SINOPEC 324006Technology Development Program of SINOPEC 325002

6 · The paper itself

Abstract

Plastic pollution caused by poly(ethylene terephthalate) (PET) highlights the urgent need for efficient biodegradation strategies. However, PET hydrolases such as DuraPETase typically exhibit limited substrate affinity for PET and insufficient operational stability. Although conventional immobilization improves enzyme stability, it often compromises catalytic activity. Here, we design a PET-targeting, orientation-controlled immobilization strategy that overcomes this traditional trade-off and enables efficient PET biodegradation. Guided by rational structural analysis, three Cys variants (R53C, R59C, R224C) were engineered for site-specific covalent attachment to a PDA@SiO

Indexed as

Enzymes, ImmobilizedPolyethylene TerephthalatesSilicon DioxideBiodegradation, EnvironmentalEnzyme StabilityKineticsEnzymes, ImmobilizedPolyethylene TerephthalatesSilicon DioxidebiodegradationDuraPETasePET-binding abilitypoly(ethylene terephthalate)sit-directed immobilization

Identifiers

PMID42197229

DOI10.3390/molecules31101675

PMCPMC13210071

What OpenQuestion holds

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LicenceCC BY

Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.