Evidence map›Paper›PMID 42196454›Full record

ArticleInternational journal of molecular sciences2026

Evaluation of a Novel Multiplex PCR Assay for Vesicular Viruses.

Giuseppe Sberna, Maria Beatrice Valli, Francesca Colavita, Fabiano Brillo, Gabriella Rozera, Claudia Minosse, Fabrizio Maggi, Eleonora Lalle

Abstract readEvaluation Study
In one paragraph

Article in International journal of molecular sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Giuseppe SbernaLaboratory of Virology and Laboratories of Biosafety, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, 00149 Rome, Italy.ORCID 0000-0003-0955-2074
Maria Beatrice ValliLaboratory of Virology and Laboratories of Biosafety, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, 00149 Rome, Italy.ORCID 0000-0001-5022-5388
Francesca ColavitaLaboratory of Virology and Laboratories of Biosafety, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, 00149 Rome, Italy.ORCID 0000-0003-0640-8667
Fabiano BrilloLaboratory of Virology and Laboratories of Biosafety, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, 00149 Rome, Italy.
Gabriella RozeraLaboratory of Virology and Laboratories of Biosafety, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, 00149 Rome, Italy.
Claudia MinosseLaboratory of Virology and Laboratories of Biosafety, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, 00149 Rome, Italy.ORCID 0000-0001-7833-9539
Fabrizio MaggiLaboratory of Virology and Laboratories of Biosafety, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, 00149 Rome, Italy.ORCID 0000-0001-7489-5271
Eleonora LalleLaboratory of Virology and Laboratories of Biosafety, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, 00149 Rome, Italy.

Funding

Italian Ministry of Health Programma Ricerca Corrente - Linea 1 on emerging and re-emerging infections
6 · The paper itself

Abstract

Differentiation and detection of viruses causing vesicular/mucocutaneous lesions are essential for patient management. This study evaluated the analytical and clinical performance of the Novaplex™ HSV-1&2/VZV/MPXV multiplex real-time PCR assay (Novaplex), designed for the simultaneous detection of monkeypox virus (MPXV), herpes virus types 1 and 2 (HSV), and varicella-zoster virus (VZV). It was compared with Singleplex PCRs as reference methods. Analytical sensitivity was assessed only for MPXV using viral stocks of clades Ia, Ib, and IIb, while clinical performances for HSV, VZV and MPXV were evaluated on 93 residual clinical samples. Novaplex discriminated clade II from clade I viruses and demonstrated low limits of detection for MPXV (clade Ia: 2.9 Log TCID

Indexed as

Herpesvirus 1, HumanHerpesvirus 2, HumanHerpesvirus 3, HumanMonkeypox virusMultiplex Polymerase Chain ReactionHumansSensitivity and Specificityherpes simplex virusHSV-1HSV-2monkeypox virusMPXVmultiplex PCRNovaplexvaricella zoster virusVZV

Identifiers

PMID42196454
PMCPMC13207437

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.