Small Extracellular Vesicle Release Following Electrical Pulse Stimulation of C2C12 Myotubes: Effects on microRNA Cargo and Myoblast Migration and Differentiation.

John S Hingle, Rhys S McColl, Ivan J Vechetti, Kathryn H Myburgh

Abstract read

In one paragraph

Article in International journal of molecular sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

John S HingleDepartment of Physiological Sciences, Stellenbosch University, Stellenbosch 7602, South Africa.ORCID 0000-0001-5429-5754

Rhys S McCollDepartment of Physiological Sciences, Stellenbosch University, Stellenbosch 7602, South Africa.ORCID 0000-0002-0116-4942

Ivan J VechettiDepartment of Nutrition and Health Sciences, College of Education and Human Sciences, University of Nebraska-Lincoln, Lincoln, NE 68583, USA.ORCID 0000-0003-1024-1011

Kathryn H MyburghDepartment of Physiological Sciences, Stellenbosch University, Stellenbosch 7602, South Africa.

Funding

National Research Foundation SARCI150212114075

6 · The paper itself

Abstract

The skeletal muscle (SkM) secretome has been widely studied since the establishment of its endocrine function. Extracellular vesicles (EVs) are the most recently identified elements of the SkM secretome. These nano-sized lipid-bound vesicles carry molecular cargo and function as a means of intercellular communication. The effect of exercise on SkM EV micro-RNA cargo (miRNAs) remains a challenge to elucidate. Electrical pulse stimulation (EPS) was applied to C2C12 myotubes at high (30 Hz) and low (2 Hz) frequencies. EVs released during 10 h of stimulation were isolated and characterized and used to treat myoblasts. Their miRNA cargo was sequenced. EVs were used to treat myoblasts (2.19 × 10

Indexed as

ExerciseExtracellular VesiclesMicroRNAsMuscle ContractionMuscle Fibers, SkeletalAnimalsCell DifferentiationCell LineCell MovementElectric StimulationMiceSecretomeMicroRNAsMirn206 microRNA, mouseC2C12 myotubeselectrical pulse stimulationextracellular vesiclesmicro-RNA cargo

Identifiers

PMID42196301

DOI10.3390/ijms27104320

PMCPMC13206931

What OpenQuestion holds

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LicenceCC BY

Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.