Characterization of Metabolite and Lipid Compositions in Lycopene-Enriched Egg Yolk Using Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry.

Xianyu You, Jishi Wang, Zeying He, Xiaoxue Yu, Xin Zhao, Liuan Li, Chaoqi Ren

Abstract read

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Article in Foods (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

7 authors.

Xianyu YouKey Laboratory of Intelligent Breeding, Ministry of Agriculture and Rural Affairs (Ministry-Province Joint Establishment), Tianjin Key Laboratory of Agricultural Animal Breeding and Health Breeding, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300392, China.

Jishi WangKey Laboratory for Environmental Factors Control of Agro-Product Quality Safety, Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China.

Zeying HeKey Laboratory for Environmental Factors Control of Agro-Product Quality Safety, Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China.

Xiaoxue YuKey Laboratory of Intelligent Breeding, Ministry of Agriculture and Rural Affairs (Ministry-Province Joint Establishment), Tianjin Key Laboratory of Agricultural Animal Breeding and Health Breeding, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300392, China.ORCID 0000-0002-8570-823X

Xin ZhaoKey Laboratory of Intelligent Breeding, Ministry of Agriculture and Rural Affairs (Ministry-Province Joint Establishment), Tianjin Key Laboratory of Agricultural Animal Breeding and Health Breeding, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300392, China.

Liuan LiKey Laboratory of Intelligent Breeding, Ministry of Agriculture and Rural Affairs (Ministry-Province Joint Establishment), Tianjin Key Laboratory of Agricultural Animal Breeding and Health Breeding, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300392, China.

Chaoqi RenKey Laboratory of Intelligent Breeding, Ministry of Agriculture and Rural Affairs (Ministry-Province Joint Establishment), Tianjin Key Laboratory of Agricultural Animal Breeding and Health Breeding, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300392, China.

Funding

Tianjin Agricultural College Young Science and Technology Talent Development Fund Project 2025QNKJ03Tianjin Outstanding Agricultural Science and Technology Commissioner Project 24ZYCGSN00060Tianjin Young Science and Technology Talent (Level 3) Project (QN20230301) QN20230301

6 · The paper itself

Abstract

This study established an untargeted metabolomic approach based on ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) to investigate differences in metabolites and lipid composition of lycopene-enriched egg yolk at different enrichment levels and conventional egg yolks. Principal component analysis and hierarchical clustering revealed clear unsupervised discrimination and separation among the control group and two treatment groups. Metabolomic analysis identified 14 differential metabolites, including amino acids, which were associated with 13 metabolic pathways such as cysteine and methionine metabolism. Lipidomic analysis revealed 48 significantly altered lipids, including phospholipids and glycerides. The results demonstrated that lycopene supplementation significantly altered the metabolic and lipid profiles of egg yolks. Specifically, lycopene enrichment upregulated phospholipid synthesis and increased the levels of antioxidant-related metabolites. This study confirms that untargeted metabolomics and lipidomics can effectively identify potential biomarkers in egg yolks with varying lycopene enrichment levels, offering new insights and a scientific basis for nutritional research and metabolic mechanism analysis of functional eggs.

Indexed as

lipidomicslycopene-enriched egg yolkuntargeted metabolomicsUPLC-QTOF-MS

Identifiers

PMID42195857

DOI10.3390/foods15101653

PMCPMC13205705

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.