Evidence map›Paper›PMID 42195516›Full record

ArticleMicromachines2026

Portable and Point-of-Care Testing Approach for Determining Soil Extracellular Enzyme Activities.

Xu Han, Fangzhou Zhang, Ruirui Chen, Weixin Wang, Yongjie Yu, Zaijiong Yi, Jingyi Yang, Bo Liu, Shilun Feng, Jun Li and 1 more

Abstract read
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In one paragraph

Article in Micromachines, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Xu HanState Key Laboratory for Development and Utilization of Forest Food Resources, Nanjing Forestry University, Nanjing 210037, China.
Fangzhou ZhangXiangfu Laboratory, Jiashan 314102, China.
Ruirui ChenState Key Laboratory for Development and Utilization of Forest Food Resources, Nanjing Forestry University, Nanjing 210037, China.ORCID 0000-0003-4582-3302
Weixin WangShanghai International Travel Healthcare Center, Shanghai Customs District, Shanghai 200335, China.
Yongjie YuSchool of Materials Engineering, Changzhou Vocational Institute of Industry Technology, Changzhou 213164, China.
Zaijiong YiShanghai International Travel Healthcare Center, Shanghai Customs District, Shanghai 200335, China.
Jingyi YangNanjing Institute of Geology and Palaeontology, Chinese Academy of Sciences, Nanjing 210008, China.
Bo LiuXiangfu Laboratory, Jiashan 314102, China.
Shilun FengXiangfu Laboratory, Jiashan 314102, China.ORCID 0000-0002-2560-2417
Jun LiShanghai International Travel Healthcare Center, Shanghai Customs District, Shanghai 200335, China.
Youzhi FengState Key Laboratory for Development and Utilization of Forest Food Resources, Nanjing Forestry University, Nanjing 210037, China.

Funding

the equipment research and development projects of the Chinese Academy of Sciences PTYQ2024BJ0007the National Key Research and Development Program of China 2023YFB3208200the National Key Research and Development Program of China 2024YFC2310202the National Natural Science Foundation of China 42577352the Science and Technology Commission of Shanghai Municipality Project XTCX-KJ-2024-038the Scientific Instruments and Equipment Development Project PTYQ2024YZ0010the Xiangfu Lab Research Project XF012023D0200
6 · The paper itself

Abstract

Soil eco-enzymes (i.e., microbial extracellular enzymes) play essential roles in terrestrial nutrient cycling and support ecosystem services. In this regard, their activities serve as indicators of soil health. However, conventional spectrophotometric and microplate fluorometric assays are often limited by lengthy reaction procedures, relatively high reagent consumption, and insufficient compatibility with complex soil matrices. In this investigation, we developed a portable, centrifugally driven microfluidic chip for the rapid and sensitive determination of multiple soil extracellular enzyme activities. This integrated platform automated sample aliquoting, reagent metering, mixing, and sedimentation, enabling the parallel measurement of eight enzymes. Such system demonstrated precise liquid control via capillary valves and high optical uniformity (<5% fluorescence variation). 4-methylumbelliferone (MUF)-based calibration exhibited strong linearity (R

Indexed as

centrifugal microfluidicsenzyme activity quantificationfluorescence detectionsoil extracellular enzymessoil health assessment

Identifiers

PMID42195516
PMCPMC13209134

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.