Design and Fabrication of a Chitosan-Based Diaphragm Digital Stethoscope for Heart Sound Acquisition.

Cardiac auscultation remains a widely used non-invasive method for assessing cardiac function; however, conventional acoustic stethoscopes are limited by subjective interpretation and lack of digital signal-handling capabilities. This study presents the design and fabrication of a chitosan-based diaphragm digital stethoscope using a biopolymer-derived acoustic interface. Chitosan was extracted from mealworm larvae shells through sequential chemical processing and subsequently processed into a glycerol-plasticized film via solution casting to obtain a flexible diaphragm. The mechanical properties of the diaphragm were evaluated to assess its suitability for acoustic applications. The diaphragm was mechanically coupled to a piezoelectric sensor and integrated into a custom 3D-printed chest piece connected to a microcontroller-based acquisition system. Heart sound signals were acquired from four conventional auscultation sites (aortic, pulmonic, tricuspid, and mitral regions). The recorded signals were processed using band-pass filtering, envelope extraction, and time-frequency analysis to visualize waveform morphology and frequency content. The signals obtained exhibited temporal and spectral features consistent with reported phonocardiography characteristics, including identifiable S1 and S2 components. These results demonstrate the feasibility of using chitosan-based diaphragm materials for heart sound acquisition in a digital stethoscope configuration, providing a low-complexity platform for further development of biopolymer-based acoustic sensing devices.