Evidence map›Paper›PMID 42194067›Full record

ArticleBiomolecules2026

Structure-Based Rational Design of TcAgo from

Xiaochen Xie, Wanping Chen, Shi Chen, Tianxin Cai, Chendi Zhang, Jie Chen, Zhenni Xu, Zhuang Li, Longyu Wang, Lixin Ma

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomolecules, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Xiaochen XieState Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan 430062, China.
Wanping ChenState Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan 430062, China.
Shi ChenState Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan 430062, China.
Tianxin CaiState Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan 430062, China.
Chendi ZhangState Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan 430062, China.
Jie ChenState Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan 430062, China.
Zhenni XuState Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan 430062, China.
Zhuang LiState Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan 430062, China.
Longyu WangState Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan 430062, China.
Lixin MaState Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan 430062, China.ORCID 0000-0002-4938-4741

Funding

Hubei Province Central Government Guided Special Fund for Local Science and Technology Development 2021CSA066National Key Research and Development Program of China 2024YFA0920800National Natural Science Foundation of China 32301221National Natural Science Foundation of China 32400513Natural Science Foundation of Hubei Province 2023AFB243Natural Science Foundation of Wuhan City 2024040701010046Project of Technological Innovation Plan in Hubei Province 2024BCA001Science and Technology Program Project of Hubei Province 2025CSA051
6 · The paper itself

Abstract

Thermophilic prokaryotic Argonaute proteins (pAgos) have emerged as powerful tools for nucleic acid manipulation, with applications in nucleic acid detection, and DNA assembly. However, their strong dependence on high-temperature catalytic activity limits their utility under moderate conditions. TcAgo, a thermophilic Argonaute nuclease from

Indexed as

Argonaute ProteinsDNAProtein EngineeringTemperatureArgonaute ProteinsDNAcold adaptationDNA-guided cleavagerational designTcAgothermophilic argonaute nucleases

Identifiers

PMID42194067
PMCPMC13204829

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.