Evidence map›Paper›PMID 42194046›Full record

ArticleBiomolecules2026

Clinical Relevance and Methodological Comparison of Anti-MDA5 Antibody Detection: A Five-Year Retrospective and Exploratory Pilot Study.

Sándor Mogyoróssy, Gábor Nagy, Zoltán Griger, Melinda Nagy-Vincze, Monika Bodoki, Dóra Csige, Tünde Tarr, Éva Zöld, György Pfliegler, Boglárka Csilla Brúgós and 9 more

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomolecules, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors.

Sándor MogyoróssyDepartment of Rheumatology, Faculty of Medicine, University of Debrecen, 4032 Debrecen, Hungary.ORCID 0009-0000-3426-5523
Gábor NagyDepartment of Laboratory Medicine, Faculty of Medicine, University of Debrecen, 4032 Debrecen, Hungary.ORCID 0000-0002-2943-2750
Zoltán GrigerDivision of Clinical Immunology, Faculty of Medicine, University of Debrecen, 4032 Debrecen, Hungary.ORCID 0000-0002-1371-7911
Melinda Nagy-VinczeDivision of Clinical Immunology, Faculty of Medicine, University of Debrecen, 4032 Debrecen, Hungary.ORCID 0000-0003-0316-3828
Monika BodokiDepartment of Rheumatology, Faculty of Medicine, University of Debrecen, 4032 Debrecen, Hungary.
Dóra CsigeDepartment of Rheumatology, Faculty of Medicine, University of Debrecen, 4032 Debrecen, Hungary.ORCID 0009-0007-5082-3944
Tünde TarrDivision of Clinical Immunology, Faculty of Medicine, University of Debrecen, 4032 Debrecen, Hungary.
Éva ZöldDivision of Clinical Immunology, Faculty of Medicine, University of Debrecen, 4032 Debrecen, Hungary.
György PflieglerCentre of Rare Diseases, Department of Internal Medicine, University of Debrecen, 4032 Debrecen, Hungary.
Boglárka Csilla BrúgósCentre of Rare Diseases, Department of Internal Medicine, University of Debrecen, 4032 Debrecen, Hungary.
Hui LuDepartment of Life Sciences, University of Bath, Bath BA2 7AY, UK.
Sarah TansleyDepartment of Life Sciences, University of Bath, Bath BA2 7AY, UK.
Péter Antal-SzalmásDepartment of Laboratory Medicine, Faculty of Medicine, University of Debrecen, 4032 Debrecen, Hungary.
Andrea DomjánDepartment of Rheumatology, Faculty of Medicine, University of Debrecen, 4032 Debrecen, Hungary.ORCID 0009-0003-3676-9911
Szilvia SzamosiDepartment of Rheumatology, Faculty of Medicine, University of Debrecen, 4032 Debrecen, Hungary.
Gabriella SzűcsDepartment of Rheumatology, Faculty of Medicine, University of Debrecen, 4032 Debrecen, Hungary.ORCID 0000-0002-0958-9531
Zoltán SzekaneczDepartment of Rheumatology, Faculty of Medicine, University of Debrecen, 4032 Debrecen, Hungary.
Ágnes HorváthDepartment of Rheumatology, Faculty of Medicine, University of Debrecen, 4032 Debrecen, Hungary.
Levente BodokiDepartment of Rheumatology, Faculty of Medicine, University of Debrecen, 4032 Debrecen, Hungary.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Anti-melanoma differentiation-associated gene 5 (anti-MDA5) antibodies are critical biomarkers in myositis, associated with distinct clinical features and prognosis. This study aimed to evaluate the proportion of anti-MDA5 positivity and compare the diagnostic performance of local immunoblotting (IB) with gold-standard immunoprecipitation (IP). We performed a retrospective analysis of 3272 physician-requested anti-MDA5 IB determinations over a five-year period (2019-2023). A subsequent exploratory pilot study of ten Hungarian patients with myositis was conducted to compare IB results with radiolabeled protein IP. Confirmatory in-house enzyme-linked immunosorbent assay (ELISA) was used to distinguish between 140 kDa bands (anti-MDA5 vs. anti-NXP2). Indirect immunofluorescence (IIF) on HEp-2 cells was also evaluated. In the retrospective cohort, 3.7% (

Indexed as

AutoantibodiesInterferon-Induced Helicase, IFIH1MyositisAdultAgedBiomarkersCross-Sectional StudiesDermatomyositisEnzyme-Linked Immunosorbent AssayFemaleHumansImmunoblottingImmunoprecipitationMaleMiddle AgedPilot ProjectsAutoantibodiesBiomarkersIFIH1 protein, humanInterferon-Induced Helicase, IFIH1anti-MDA5dermatomyositisidiopathic inflammatory myopathyimmunoblotimmunoprecipitation

Identifiers

PMID42194046
PMCPMC13204031

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.