ArticleCells2026
A Novel Selective Strategy for Bioactive Limbal Stem Cells Primary Culture Using Deep Cryopreservation and IL-1β Precondition.
Article in Cells, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Limbal stem cell (LSC) transplantation is an important treatment for limbal stem cell deficiency (LSCD), but low efficacy in maintaining LSC stemness during in vitro expansion greatly affects its wider application. The primary contributing factors include a low proportion of stem cells and the lack of a stable, supportive microenvironment over prolonged culture. Rabbit corneal tissues preserved under deep cryogenic conditions for more than six months retain viable limbal stem cells (LSCs), and primary LSCs isolated from these tissues exhibit robust stem cell characteristics. It is noteworthy that the NLRP3/Caspase-1/IL-1β signaling axis was activated in corneal epithelial cells, and outer limbal layers preserved for one or two years. Based on these findings, a combined strategy integrating deep cryopreservation with IL-1β induction was established for the processing of limbal tissues. The combined cryogenic and IL-1β preconditioning yielded primary LSCs with maintained p63
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