Oridonin Suppresses Colorectal Cancer Growth In Vitro and In Vivo: Evidence from Integrated Transcriptomic and Proteomic Profiling.

Menglong Xu, Yongchao Li, Wenqiang Sun, Haocheng Guan, Tinghui Wu, Shuwei Li

Abstract read

In one paragraph

Article in Current issues in molecular biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Menglong XuDepartment of Biochemistry and Molecular Biology, College of Life Science and Technology, the State Key Laboratory Incubation Base for Conservation and Utilization of Bio-Resource in the Tarim Basin of the Xinjiang Production and Construction Corps, Tarim University, Alar 843300, China.

Yongchao LiDepartment of Biochemistry and Molecular Biology, College of Life Science and Technology, the State Key Laboratory Incubation Base for Conservation and Utilization of Bio-Resource in the Tarim Basin of the Xinjiang Production and Construction Corps, Tarim University, Alar 843300, China.ORCID 0000-0003-2016-1824

Wenqiang SunDepartment of Biochemistry and Molecular Biology, College of Life Science and Technology, the State Key Laboratory Incubation Base for Conservation and Utilization of Bio-Resource in the Tarim Basin of the Xinjiang Production and Construction Corps, Tarim University, Alar 843300, China.

Haocheng GuanDepartment of Biochemistry and Molecular Biology, College of Life Science and Technology, the State Key Laboratory Incubation Base for Conservation and Utilization of Bio-Resource in the Tarim Basin of the Xinjiang Production and Construction Corps, Tarim University, Alar 843300, China.

Tinghui WuSchool of Medical Technology, Xinjiang Hetian College, Hetian 848000, China.

Shuwei LiDepartment of Biochemistry and Molecular Biology, College of Life Science and Technology, the State Key Laboratory Incubation Base for Conservation and Utilization of Bio-Resource in the Tarim Basin of the Xinjiang Production and Construction Corps, Tarim University, Alar 843300, China.ORCID 0000-0002-4160-9133

Funding

Observation and Application of the Therapeutic Effect of Traditional Chinese Medicine Prescriptions from Authentic Medicinal Materials in Xinjiang in the Treatment of Burns and Scalds 2024RC012Treatment of Burns and Scalds and Promotion of Full-Functional Skin Reconstruction Using Traditional Chinese Medicine Prescriptions from Characteristic Plants in Southern Xinjiang 2024ZR-009Xinjiang Uyghur Autonomous Region Department of Science and Technology and the Xinjiang Production and Construction Corps Science and Technology Bureau, "Tianshan Talents"-Science and Technology Innovation Leading Talents Training Projec 2023TSYCLJ0057

6 · The paper itself

Abstract

Colorectal cancer (CRC) remains a major cause of cancer-related mortality worldwide, and effective therapeutic options for advanced disease are still limited. Oridonin (ORI), a naturally derived diterpenoid compound, has shown anti-tumor activity in several malignancies, but its molecular mechanisms in CRC remain incompletely understood. In this study, the anti-cancer effects of ORI were evaluated in HT-29 and HCT116 colorectal cancer cells using in vitro assays, integrated transcriptomic and proteomic analyses, Western blotting, and an HT-29 xenograft model. ORI reduced cell viability in a time- and concentration-dependent manner, induced G1-phase cell cycle arrest, increased cell death, and reduced wound closure under the tested in vitro conditions. Integrated omics analyses in HT-29 cells identified extensive alterations in gene and protein expression, with significant enrichment of pathways related to cell cycle regulation and apoptosis. Western blotting further showed that ORI increased the expression of BAX, BID, CYCS, and CASP3 while decreasing BCL2 expression. In vivo, ORI significantly inhibited tumor growth in nude mice bearing HT-29 xenografts. These findings indicate that ORI suppresses CRC growth through coordinated regulation of cell cycle progression and apoptosis and suggest that ORI may serve as a potential therapeutic candidate for colorectal cancer.

Indexed as

apoptosiscolorectal canceroridoninproteomictranscriptomic

Identifiers

PMID42193045

DOI10.3390/cimb48050440

PMCPMC13204077

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