Evidence map›Paper›PMID 42191998›Full record

ArticleMolecular genetics and genomics : MGG2026

Genome-wide identification, in-silico characterization, and expression analysis of polygalacturonases in different fungal species.

Laiba Amin, Imran Zafar, Adil Jamal, Syed M Safeer Mehdi Bokhari, Fayez Saeed Bahwerth, Muhammad Noman

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Article in Molecular genetics and genomics : MGG, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Laiba AminPlant Biotechnology Group, National University of Sciences and Technology, Islamabad, Pakistan.
Imran ZafarDepartment of Biochemistry and Biotechnology, Faculty of Sciences, The University of Faisalabad, Faisalabad, 38000, Punjab, Pakistan.
Adil JamalDepartment of Biochemistry and Biotechnology, Faculty of Sciences, The University of Faisalabad, Faisalabad, 38000, Punjab, Pakistan. adiljamalcemb@gmail.com.
Syed M Safeer Mehdi BokhariDepartment of Biochemistry and Biotechnology, Faculty of Sciences, The University of Faisalabad, Faisalabad, 38000, Punjab, Pakistan.
Fayez Saeed BahwerthHera General Hospital, Makkah, 21955, Saudi Arabia.
Muhammad NomanDepartment of Medical Lab Sciences, Faculty of Allied Health Sciences and Medicine, The University of Faisalabad, Faisalabad, 38000, Punjab, Pakistan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Polygalacturonases (PGs) are key enzymes that hydrolyze pectin, a complex polysaccharide in plant cell walls, with essential roles in biological processes and industrial applications. This study presents a comprehensive genome-wide identification and in-silico characterization of PGs from four fungal species: Aspergillus oryzae, Aspergillus flavus, Neurospora crassa, and Rhizoctonia solani. A total of 44 PG protein sequences were retrieved from the NCBI database, confirming the presence of the Glyco_hydro_28 (GH28) domain, which is essential for pectin hydrolysis. Phylogenetic analysis using the Neighbor-Joining method revealed four major clades (A, B, C, D), with A. flavus and A. oryzae sharing a close evolutionary relationship (bootstrap support = 93%). Gene structure analysis revealed that A. flavus (Af1) and A. oryzae (Ao1) each have one exon, while Neurospora crassa (NSc1) contains one intron. Ten conserved motifs were identified, with Motif 1 present in Rz1, Rz2, and Rz5, and Motif 2 in all sequences except Ao15 and Ao16. Chromosomal mapping indicated species-specific gene distributions, with A. oryzae showing genes spread across five chromosomes. Gene expression analysis of A. oryzae under various growth conditions revealed 20 differentially expressed genes (DEGs), including 10 upregulated (e.g., Gene_Ao1, LFC = 2.50, FDR = 0.00450) and 10 downregulated (e.g., Gene_AO9, LFC = -2.87, FDR = 1.1e-05). Pathway enrichment analysis highlighted significant involvement of PGs in apoptosis (FDR = 1.2e0

Indexed as

PolygalacturonaseAspergillus flavusAspergillus oryzaeChromosome MappingComputer SimulationFungal ProteinsGene Expression Regulation, FungalGenome, FungalNeurospora crassaPhylogenyRhizoctoniaFungal ProteinsPolygalacturonaseFungal speciesGene expression analysisIn-silico characterizationPolygalacturonases (PGs)Protein-protein interaction (PPI) network

Identifiers

PMID42191998

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