ArticleThe ISME journal2026
Aesculetin-mediated recruitment of denitrifying microbiota by resistant rapeseed suppresses Plasmodiophora brassicae via nitrate depletion.
Article in The ISME journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
15 authors.
Funding
Abstract
Clubroot, caused by the protist pathogen Plasmodiophora brassicae, is a major threat to cruciferous crop production worldwide. Although plant microbiota is known to influence disease outcomes, the mechanisms underlying microbiota-mediated resistance remain unclear. Here, we investigated the role of plant microbiota in clubroot resistance using two Chinese rapeseed cultivars carrying resistance genes introduced through breeding and their susceptible parental lines. Microbiome profiling revealed that P. brassicae infection altered root and rhizosphere bacterial communities, with resistant cultivars displaying distinct assemblages. Functional prediction indicated an enrichment of denitrifying bacteria in the roots of resistant plants following pathogen challenge. Key denitrifying strains were isolated and assembled into a synthetic microbial community (SynCom18), which significantly suppressed clubroot development under both controlled and field conditions. In addition to reducing disease severity, microbial treatments improved agronomic traits, including yield and seed quality. Mechanistic analysis revealed a positive correlation between soil nitrate levels and disease severity. Denitrifying strains and SynCom18 likely suppressed the development of P. brassicae and enhanced plant immunity by reducing soil nitrate levels by ~39.4%. Metabolomic profiling revealed that aesculetin, as a dominant metabolite that is produced by resistance roots and excreted into the rhizosphere to recruit denitrifying bacteria. Our findings show that pathogen-infected clubroot-resistant rapeseed cultivars secrete aesculetin to recruit nitrate-depleting bacteria for resistance against P. brassicae. This study elucidates a tripartite microbiota-pathogen-soil nutrient interaction and provides a sustainable biocontrol strategy for cruciferous crops.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.