ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2026
Optimization of stool culture protocol in a tertiary hospital in Bangkok, Thailand: a prospective cohort study.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
purposeStool culture protocols are resource-intensive, and the necessity of specific selective media is frequently debated. We aimed to determine the prevalence of bacterial enteric pathogens in a Thai tertiary hospital and optimize routine culture protocols by evaluating various diagnostic methods.
methodsWe evaluated 597 diarrheal stool samples using standard protocols for Salmonella enterica, Shigella spp., Vibrio spp., Aeromonas spp., and Plesiomonas shigelloides. Additional protocols evaluated the detection of Campylobacter spp. (selective medium and membrane filtration) and enteric Yersinia spp. (cefsulodin-irgasan-novobiocin [CIN] agar). To estimate S. enterica method sensitivities, we applied Bayesian latent class analysis (LCA). Additionally, analytical limit of detection (LOD) studies using spiked stool matrices were performed for S. enterica, Campylobacter spp., and Yersinia spp.
resultsEnteric pathogens were isolated from 12.7% of the samples, predominantly S. enterica (10.4%) and Campylobacter spp. (2.2%); no Yersinia spp. were detected. For S. enterica, LCA estimated sensitivities of Gram-negative (GN) broth (97.4%) and modified semisolid Rappaport Vassiliadis (MSRV) agar (89.4%), significantly outperforming other solid media. Campylobacter membrane filtration and selective agar showed near-perfect clinical agreement (99.8%); however, spiked LOD testing revealed that selective agar was 10
conclusionGN broth and MSRV agar are needed to maximize S. enterica yield. Routine CIN agar for Yersinia spp. is not cost-efficient, and Campylobacter selective agar should replace membrane filtration to prevent false-negative reporting in low-shedding patients.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.