ArticleVaccines2026
Impact of Media and Induction Strategy on Physicochemical Characteristics and Immunogenicity of Recombinant fHbp-PorA Chimeric Protein: A Promising Meningococcal B Vaccine Candidate Produced in
Article in Vaccines, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
BACKGROUND/
objectiveApart from the attributes such as cost, yields and consistency that define the feasibility of a manufacturing process, physicochemical and immunological quality traits equally signify the functionality of a biological product. The present study investigates one such promising Meningococcal B vaccine candidate, a chimeric fHbp-PorA protein in
resultsGrowth in the CD media resulted in several-fold-higher biomass compared to that in CM media in a short cultivation time; however, more than a third of the expressed protein remained in an insoluble state. Meanwhile, almost all of the expressed protein with CM media was recovered in soluble form. Moreover, purification of the unprocessed tagged protein and recovery of chimeric protein (tag removed) resulted in 75% greater yield in CM media when compared to CD media. The final chimeric proteins obtained from each medium varied significantly in their physicochemical characteristics, including their epitope projection and CD spectra. The results of
conclusionsThe outcomes demonstrate that complex media with galactose-induced expression not only show higher productivity but also exhibit superior quality attributes, qualifying their reliable use in the manufacturing process of this promising vaccine candidate.
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