Protoplasts Isolation and Transient Transformation System Optimization for Poplar 84K (

Chao Yu, Huimin Yu, Yirong Rui, Meiling Wang

Abstract read

In one paragraph

Article in Biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Chao YuCollaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas, College of Horticulture, Zhejiang A&F University, Hangzhou 311300, China.ORCID 0000-0003-1700-684X

Huimin YuCollaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas, College of Horticulture, Zhejiang A&F University, Hangzhou 311300, China.

Yirong RuiCollaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas, College of Horticulture, Zhejiang A&F University, Hangzhou 311300, China.

Meiling WangCollege of Agriculture and Biology, Liaocheng University, Liaocheng 252000, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In poplar, the protracted stable genetic transformation procedure constrains rapid gene functional analyses. To address this limitation, we optimized a protocol for the high-yield isolation and efficient transient transformation of protoplasts from leaves of tissue-cultured poplar 84K (

Indexed as

poplar 84Kprotoplast

Identifiers

PMID42187742

DOI10.3390/biology15100780

PMCPMC13203830

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.