ArticleChembiochem : a European journal of chemical biology2026
Enhanced Enzyme Cascade Reactions Through Coencapsulation in Biocompatible Silica Nanoconfinement.
Article in Chembiochem : a European journal of chemical biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Enhanced Enzyme Cascade Reactions Through Coencapsulation in Biocompatible Silica Nanoconfinement.Chembiochem : a European journal of chemical biology · 2026Article
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Authors and funding
11 authors.
Funding
Abstract
Enzyme cascade reactions, where the product of one enzyme serves as the substrate for another, are fundamental to cellular metabolism and essential for constructing efficient multi-enzyme systems. Spatial confinement of sequential enzymes can enhance cascade efficiency through proximity effects and substrate channeling, mimicking the organization of natural metabolic pathways. Here, we coencapsulated glucose oxidase (GOX) and horseradish peroxidase (HRP) within silica nanocapsules (SiNCs) using an enzyme-friendly synthesis method. The encapsulated enzymes retained high catalytic activity, and Michaelis-Menten kinetics analysis revealed decreased Km for GOX, indicating enhanced substrate affinity under confinement. The coencapsulated system demonstrated faster cascade kinetics compared to spatially separated enzyme configurations, providing direct evidence for the beneficial effects of enzyme proximity on intermediate transfer. Enhanced thermal stability of confined enzymes was also observed, presumably due to preserved hydration shells. This platform enables quantitative studies on enzyme behavior under nanoscale confinement, offering insights into the biochemical principles governing multi-enzyme cascade systems in both natural and artificial environments.
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