ArticleChembiochem : a European journal of chemical biology2026
Improvement of cDNA TRAP Display via Optimization of Puromycin Linker Design for Enhanced Discovery of Antibody-Like Proteins.
Article in Chembiochem : a European journal of chemical biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
cDNA TRAP display is a high-speed in vitro selection method for obtaining antibody-like proteins (ALPs) with desired functions, enabling selection even under harsh conditions. However, since its development, this method has not been applied to practical selection because of the low formation efficiency of the ALP-puromycin linker/mRNA complex (display efficiency), which is a key determinant of library diversity. In this study, we aimed to improve display efficiency by optimizing the puromycin linker design. Specifically, we evaluated modifications to the nucleotides at the 5' end and attachment position of the puromycin moiety at the 3' end, which resulted in a 1.8-fold increase in display efficiency. These findings provide insights into enhancing the performance of cDNA TRAP display and are expected to facilitate future ALP discovery.
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