Evidence map›Paper›PMID 42186450›Full record

ArticleFrontiers in toxicology2026

PFOA/PFOS induce ferroptosis in bladder epithelial cells through inhibition of ACSL4 ubiquitination.

Hao Xu, Zhihua Ye, Juan Liu, Mengwei Liu, Qinghua Hu, Yi Liu, Qiankun Zhang, Jian Ou, Xin Shen, Xiaogang Chen

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Article in Frontiers in toxicology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Hao Xu *Department of Urology, Huangshi Central Hospital, Affiliated Hospital of Hubei Polytechnic University, Huangshi, Hubei, China.
Zhihua Ye *Department of Urology, Huangshi Central Hospital, Affiliated Hospital of Hubei Polytechnic University, Huangshi, Hubei, China.
Juan Liu *Hubei Key Laboratory of Kidney Disease Pathogenesis and Intervention, Huangshi, Hubei, China.
Mengwei LiuDepartment of Urology, Huangshi Central Hospital, Affiliated Hospital of Hubei Polytechnic University, Huangshi, Hubei, China.
Qinghua HuDepartment of Urology, Huangshi Central Hospital Graduate Joint Training Base, School of Medicine, Wuhan University of Science and Technology, Huangshi, Hubei, China.
Yi LiuDepartment of Urology, Huangshi Central Hospital Graduate Joint Training Base, School of Medicine, Wuhan University of Science and Technology, Huangshi, Hubei, China.
Qiankun ZhangDepartment of Urology, Huangshi Central Hospital Graduate Joint Training Base, School of Medicine, Wuhan University of Science and Technology, Huangshi, Hubei, China.
Jian OuHubei Key Laboratory of Kidney Disease Pathogenesis and Intervention, Huangshi, Hubei, China.
Xin ShenHubei Key Laboratory of Kidney Disease Pathogenesis and Intervention, Huangshi, Hubei, China.
Xiaogang ChenDepartment of Urology, Huangshi Central Hospital, Affiliated Hospital of Hubei Polytechnic University, Huangshi, Hubei, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Perfluoroalkyl and polyfluoroalkyl substances (PFAS) are persistent environmental pollutants, but their role in bladder epithelial injury remains unclear. Methods: SV-HUC-1 cells were exposed to PFOA and PFOS. Cell viability, lipid peroxidation, and ROS levels were assessed using CCK-8 assay, flow cytometry, and transmission electron microscopy. Protein expression and ubiquitination were analyzed by Western blotting and molecular docking. Results: PFOA and PFOS induced ferroptosis in bladder epithelial cells, characterized by increased lipid peroxidation and ROS levels. Mechanistically, PFOA/PFOS inhibited ACSL4 ubiquitination at K593 and K690, leading to its stabilization and promoting ferroptosis. Knockdown of ACSL4 significantly reversed these effects. Discussion: These findings reveal a novel mechanism by which PFAS induce ferroptosis via regulation of ACSL4 ubiquitination, providing new insights into PFAS-induced bladder toxicity.

Indexed as

ACSL4bladder epithelial cellferroptosispfoaPFOsubiquitination

Identifiers

PMID42186450
PMCPMC13198926

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.