Modified skills of trypsin-digested retinal vasculature mount preparation and string vessels observation.

Mi Song, Haoyu Li, Benteng Ma, Baihua Chen

Abstract read

In one paragraph

Article in BMC ophthalmology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

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0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Mi SongDepartment of Ophthalmology, The Second Xiangya Hospital, Central South University, No.139 Middle Renmin Road, Changsha, Hunan, 410011, China.

Haoyu LiDepartment of Ophthalmology, The Second Xiangya Hospital, Central South University, No.139 Middle Renmin Road, Changsha, Hunan, 410011, China.

Benteng MaDepartment of Ophthalmology, The Second Xiangya Hospital, Central South University, No.139 Middle Renmin Road, Changsha, Hunan, 410011, China.

Baihua ChenDepartment of Ophthalmology, The Second Xiangya Hospital, Central South University, No.139 Middle Renmin Road, Changsha, Hunan, 410011, China. chenbaihua2017@csu.edu.cn.

Funding

National Natural Science Foundation of China 82171030 and NO. 81870678Natural Science Foundation of Hunan Province 2021JJ30925

6 · The paper itself

Abstract

Trypsin-digested retinal vasculature mount is a key method to study vascular retinopathy. However, its failure rate and difficulty maintaining the vascular network's integrity limit its wide application. String vessels occur in the vasculature of different tissues in many species. However, the definition and function of string vessels are full of controversy. This study aimed to explore the skills of preparing complete and repeatable retinal vasculature mounts and further understand the string vessel through observation. We found that the integrity of the retinal vascular network was successfully protected by using toothless micro forceps to clamp the internal limiting membrane and using glass rods to lift the vascular network from the water surface. This modification will not waste the hard-won samples. We observed the different states of the string vessels and hypothesized that they might participate in angiogenesis during vascular development or under pathological conditions.

Indexed as

Retinal VesselsTrypsinAnimalsTrypsinAcellular capillaryAngiogenesisInterpapillary bridgeRetinal vasculature mountString vesselTrypsin digestion

Identifiers

PMID42185836

DOI10.1186/s12886-026-04951-1

PMCPMC13404314

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.