Evidence map›Paper›PMID 42183359›Full record

ArticleResearch square2026

Three-metal-ion catalysis by ribonuclease P holoenzyme: a new mechanistic insight into transfer RNA maturation.

Yun-Tzai Lee, Maximilia F S Degenhardt, Ilias Skeparnias, Szu-Yun Chen, Bapurao A Bhoge, Sergey G Tarasov, Marzena A Dyba, Dan Shi, Jinwei Zhang, Jason R Stagno and 1 more

Abstract readPreprint
In one paragraph

Article in Research square, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Yun-Tzai LeeProtein-Nucleic Acid Interaction Section, Center for Structural Biology, National Cancer Institute, Frederick, Maryland 21702, USA.ORCID https://orcid.org/0000-0001-8123-6207
Maximilia F S DegenhardtProtein-Nucleic Acid Interaction Section, Center for Structural Biology, National Cancer Institute, Frederick, Maryland 21702, USA.ORCID https://orcid.org/0000-0003-4522-377X
Ilias SkeparniasLaboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892, USA.ORCID https://orcid.org/0000-0003-1188-529X
Szu-Yun ChenProtein-Nucleic Acid Interaction Section, Center for Structural Biology, National Cancer Institute, Frederick, Maryland 21702, USA.
Bapurao A BhogeProtein-Nucleic Acid Interaction Section, Center for Structural Biology, National Cancer Institute, Frederick, Maryland 21702, USA.
Sergey G TarasovBiophysics Resource, Center for Structural Biology, National Cancer Institute, Frederick, Maryland 21702, USA.
Marzena A DybaBiophysics Resource, Center for Structural Biology, National Cancer Institute, Frederick, Maryland 21702, USA.
Dan ShiCryo-EM Facility, Center for Structural Biology, National Cancer Institute, Frederick, Maryland 21702, USA.
Jinwei ZhangLaboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892, USA.ORCID https://orcid.org/0000-0002-2114-173X
Jason R StagnoProtein-Nucleic Acid Interaction Section, Center for Structural Biology, National Cancer Institute, Frederick, Maryland 21702, USA.ORCID https://orcid.org/0000-0002-6464-7829
Yun-Xing WangProtein-Nucleic Acid Interaction Section, Center for Structural Biology, National Cancer Institute, Frederick, Maryland 21702, USA.ORCID https://orcid.org/0000-0002-2175-0148

Funding

Search for the Structural Basis of Biomacromolecular Function and ActivityZIABC010379 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI WANG, YUN XING M · 2009 to 2025
$30.4M
Noncoding RNA structures and interactions in cellular stress responses and immunityZIADK075136 · NIDDK · NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES · PI ZHANG, JINWEI · 2016 to 2025
$23.2M
SAXS FacilityZICBC011535 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI WANG, YUN XING M · 2014 to 2025
$7.5M
NCI X-ray free electron laser (XFEL) initiativeZIABC011669 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI WANG, YUN XING M · 2015 to 2024
$5.0M
Intramural NIH HHS ZIA BC010379Intramural NIH HHS ZIA BC011669Intramural NIH HHS ZIA DK075136Intramural NIH HHS ZIC BC011535
6 · The paper itself

Abstract

Ribonuclease P (RNase P) is an essential metalloenzyme responsible for tRNA 5' maturation across all three domains of life. The catalytic mechanism, particularly the number of divalent metal ions and the role of its protein component, remains unresolved. We determined cryo-EM structures of

Identifiers

PMID42183359
PMCPMC13193133

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.