Fecal Microbiota Transplantation From Healthy Donors Reduces Glycemic Variability in Streptozotocin-Induced Diabetic Rats via Enhanced Hepatic Glycogen Synthesis.

Background: Blood glucose fluctuations in patients with brittle diabetes have been a problem for clinicians. A recent study found that transplanting the intestinal flora of healthy people to patients with brittle diabetes can improve their blood glucose fluctuations. However, the underlying mechanism remains unclear. Methods: Streptozotocin-induced diabetic rats were assigned to receive fecal microbiota transplantation (FMT) from healthy donors or to remain untreated, while normal rats received FMT from brittle diabetes donors or remained untreated. Groups included the normal control group (NC group), the diabetic group (DM group), normal rats with FMT from brittle diabetic patients (NC-DMFMT group), and diabetic rats with FMT from normal individuals (DM-NCFMT group). Blood glucose variability, rat liver glucokinase, and glycogen levels, as well as intestinal short-chain fatty acid content, were detected in each group of rats. Gut microbiota composition was analyzed using 16S rDNA sequencing. Results: Compared with the standard deviation of blood glucose (SDBG) (1.664 ± 0.427 mmol/L) in the NC group, that of the DM group (6.879 ± 1.475 mmol/L) was higher. However, the DM-NCFMT group reduced SDBG (4.387 ± 0.619 mmol/L) vs. the DM group ( Conclusion: Normal individual fecal microbiota transplantation improves glucose variability in DM rats, potentially mediated by enhanced hepatic glycogen synthesis.