Evidence map›Paper›PMID 42182941›Full record

ArticleJournal of clinical biochemistry and nutrition2026

Non-thermal plasma-activated methionine-containing Ringer's solution enhances cytotoxicity by increasing oxidative stress.

Kosei Fujisawa, Sakura Tanahashi, Kyoka Katagiri, Soo Takasu, Tomohiro Otsuka, Tetsuro Kamiya, Kenji Ishikawa, Yukihiro Esaka, Hirokazu Hara

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In one paragraph

Article in Journal of clinical biochemistry and nutrition, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Kosei FujisawaLaboratory of Clinical Pharmaceutics, Gifu Pharmaceutical University, 1-25-4 Daigaku-nishi, Gifu 501-1196, Japan.
Sakura TanahashiLaboratory of Clinical Pharmaceutics, Gifu Pharmaceutical University, 1-25-4 Daigaku-nishi, Gifu 501-1196, Japan.
Kyoka KatagiriLaboratory of Pharmaceutical Analytical Chemistry, Gifu Pharmaceutical University, 1-25-4 Daigaku-nishi, Gifu 501-1196, Japan.
Soo TakasuLaboratory of Pharmaceutical Analytical Chemistry, Gifu Pharmaceutical University, 1-25-4 Daigaku-nishi, Gifu 501-1196, Japan.
Tomohiro OtsukaLaboratory of Clinical Pharmaceutics, Gifu Pharmaceutical University, 1-25-4 Daigaku-nishi, Gifu 501-1196, Japan.
Tetsuro KamiyaLaboratory of Clinical Pharmaceutics, Gifu Pharmaceutical University, 1-25-4 Daigaku-nishi, Gifu 501-1196, Japan.
Kenji IshikawaCenter for Low-temperature Plasma Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya-shi, Aichi 464-8601 Japan.
Yukihiro EsakaLaboratory of Pharmaceutical Analytical Chemistry, Gifu Pharmaceutical University, 1-25-4 Daigaku-nishi, Gifu 501-1196, Japan.
Hirokazu HaraLaboratory of Clinical Pharmaceutics, Gifu Pharmaceutical University, 1-25-4 Daigaku-nishi, Gifu 501-1196, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Non-thermal plasma (NTP) is an ionized gas composed of electrons, ions, radicals, and UV. NTP-irradiated solutions induce reactive oxygen species (ROS)-dependent cell death. NTP irradiation of solutions containing methionine (Met) has been reported to enhance the cytotoxic effects of NTP; however, the exact mechanism by which NTP-irradiated Met solutions exhibit cytotoxicity is not fully understood. In this study, we aimed to elucidate the mechanism using the human breast cancer cell line MDA-MB-231 cells. NTP-irradiated Met-containing acetate Ringer's solution (Met/PAA) potently induced cytotoxicity compared with NTP-irradiated acetate Ringer's solution (PAA). Catalase-treated Met/PAA induced cytotoxicity to the same extent, suggesting that NTP irradiation might generate a novel cytotoxic substance distinct from ROS. Catalase-treated Met/PAA decreased intracellular glutathione (GSH) levels, increased ROS production, and caused mitochondrial dysfunction. When catalase-treated Met/PAA was pretreated with antioxidants, GSH and

Indexed as

cytotoxicitymethioninenon-thermal plasmaplasma-activated solutionreactive oxygen species

Identifiers

PMID42182941
PMCPMC13193816

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.