Evidence map›Paper›PMID 42182312›Full record

ArticlebioRxiv : the preprint server for biology2026

Tunable gene expression in zebrafish using RiboSCALE.

Thomas P Rynes, Eiman A Osman, Maureen McKeague, Karen Mruk

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Thomas P RynesDepartment of Pharmacology and Toxicology, Brody School of Medicine, East Carolina University, Greenville, NC, USA.ORCID 0009-0005-5802-2354
Eiman A OsmanDepartment of Chemistry, Faculty of Science, McGill University, Montreal, QC H3A 0B8, Canada.ORCID 0009-0003-7676-2474
Maureen McKeagueDepartment of Chemistry, Faculty of Science, McGill University, Montreal, QC H3A 0B8, Canada.ORCID 0000-0002-3750-6027
Karen MrukDepartment of Pharmacology and Toxicology, Brody School of Medicine, East Carolina University, Greenville, NC, USA.ORCID 0000-0003-4560-4951

Funding

RNA-based tools for developmental and regenerative biologyR21GM143565 · NIGMS · UNIVERSITY OF WYOMING · PI MCKEAGUE, MAUREEN HOPE, MRUK, KAREN · 2021 to 2022
$356k
Elucidating the role of kcnn1 in recovery from spinal cord injuryR03NS136719 · NINDS · EAST CAROLINA UNIVERSITY · PI MRUK, KAREN · 2024 to 2025
$151k
NIGMS NIH HHS R21 GM143565NINDS NIH HHS R03 NS136719
6 · The paper itself

Abstract

Chemogenetic tools enable conditional control of gene expression during embryonic development and regeneration. However, many conditional tools induce constitutive or one-way activity precluding temporal resolution of gene function or require the use of multiple transgenic lines. We developed an RNA-based chemogenetic approach to induce gene expression in zebrafish embryos and larvae. We demonstrate that a gene of interest can be turned on in a time-dependent and concentration-dependent manner. Using this approach, we have characterized two different aptamers for future investigation.

Indexed as

aptamerChemogeneticsribozymezebrafish

Identifiers

PMID42182312
PMCPMC13192851

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.