ArticlebioRxiv : the preprint server for biology2026
Heterogeneous reconstruction algorithms for cryoEM achieve limited particle classification accuracy on real benchmark datasets.
Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
The emergence of single-particle cryoEM as a powerful method for structure determination has in large part been fueled by its ability to resolve both single static structures and complex conformational landscapes. Indeed, modern approaches to the heterogeneous reconstruction task can resolve 100s-1,000s of different maps from a single cryoEM dataset. How accurate these algorithms are, however, has proven difficult to rigorously assess, due to a lack of suitable benchmark datasets containing both realistic noise features and ground-truth labels. To address this obstacle, we recently developed a series of benchmark datasets that leverage the targeting power of Cas9 and the programmable heterogeneity of DNA to newly offer access to ground-truth per-particle structural labels in real data. Here, we challenged two popular heterogeneous reconstruction algorithms with mixed particle stacks resampled
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.