Evidence map›Paper›PMID 42182160›Full record

ArticlebioRxiv : the preprint server for biology2026

Expansion Revealing of Pathology Resolves Nanostructures Associated with Inflammatory Phenotypes in COVID-19 Decedent Human Brain Tissue.

Alice E Stanton, Jinyoung Kang, Joel W Blanchard, Carles A Boix, Margaret E Schroeder, Youngmi Lee, Hanquan Su, Shiwei Wang, Eunah Yu, Amauche Emenari and 11 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors.

Alice E StantonKoch Institute, MIT, Cambridge, MA, 02139, USA.
Jinyoung KangMcGovern Institute for Brain Research, MIT, Cambridge, MA, 02139, USA.ORCID 0000-0001-6116-3477
Joel W BlanchardPicower Institute for Learning and Memory, MIT, Cambridge, MA, 02139, USA.
Carles A BoixComputer Science and Artificial Intelligence Laboratory, MIT, Cambridge, MA, 02139, USA.
Margaret E SchroederMcGovern Institute for Brain Research, MIT, Cambridge, MA, 02139, USA.
Youngmi LeeMcGovern Institute for Brain Research, MIT, Cambridge, MA, 02139, USA.
Hanquan SuWyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, 02115, USA.
Shiwei WangMcGovern Institute for Brain Research, MIT, Cambridge, MA, 02139, USA.
Eunah YuMcGovern Institute for Brain Research, MIT, Cambridge, MA, 02139, USA.
Amauche EmenariDepartment of Brain and Cognitive Sciences, MIT, Cambridge, MA, 02139, USA.
Zhuyu PengDepartment of Brain and Cognitive Sciences, MIT, Cambridge, MA, 02139, USA.
Emre AgbasPicower Institute for Learning and Memory, MIT, Cambridge, MA, 02139, USA.
Oyku CeritPicower Institute for Learning and Memory, MIT, Cambridge, MA, 02139, USA.
Demian ParkMcGovern Institute for Brain Research, MIT, Cambridge, MA, 02139, USA.
Ruihan ZhangMedia Arts and Sciences, MIT, Cambridge, MA, 02139, USA.
David A BennettRush Alzheimer's Disease Center, Rush University Medical Center, Chicago, IL, 60612, USA.
Peng YinWyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, 02115, USA.
Manolis KellisComputer Science and Artificial Intelligence Laboratory, MIT, Cambridge, MA, 02139, USA.
Robert LangerKoch Institute, MIT, Cambridge, MA, 02139, USA.
Edward BoydenKoch Institute, MIT, Cambridge, MA, 02139, USA.
Li-Huei TsaiPicower Institute for Learning and Memory, MIT, Cambridge, MA, 02139, USA.ORCID 0000-0003-1262-0592

Funding

The impact of HIV viral diversity and cellular immunity on HIV pathogenesisP30AI060354 · NIAID · HARVARD UNIVERSITY (MEDICAL SCHOOL) · PI RAJESH T GANDHI · 2004 to 2026
$94.4M
SUPPLEMENT TO RUSH ALZHEIMERS DISEASE CENTER COREP30AG010161 · NIA · RUSH UNIVERSITY MEDICAL CENTER · PI LEURGANS, SUE E. · 1991 to 2020
$49.1M
EPIDEMIOLOGY OF NEURAL RESERVE AND NEUROBIOLOGY IN AGINGR01AG017917 · NIA · RUSH UNIVERSITY MEDICAL CENTER · PI BENNETT, DAVID ALAN · 2001 to 2023
$43.3M
Rush Alzheimer's Disease Research CenterP30AG072975 · NIA · RUSH UNIVERSITY MEDICAL CENTER · PI Lisa L Barnes, Julie A. Schneider · 2021 to 2026
$24.7M
RISK FACTORS, PATHOLOGY, AND CLINICAL EXPRESSIONS OF ADR01AG015819 · NIA · RUSH UNIVERSITY MEDICAL CENTER · PI BENNETT, DAVID ALAN · 1998 to 2024
$21.4M
BRAIN-ICU-2 Study: Bringing to Light the Risk Factors And Incidence of Neuropsychological Dysfunction (Dementia) in ICU Survivors, 2nd StudyR01AG058639 · NIA · VANDERBILT UNIVERSITY MEDICAL CENTER · PI ELY, E WESLEY, JEFFERSON, ANGELA L. · 2019 to 2024
$18.3M
Expansion MicroscopyR01EB024261 · NIBIB · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S. · 2017 to 2025
$4.6M
High-throughput approaches to local and long-range synaptic connectivityRF1MH123403 · NIMH · COLD SPRING HARBOR LABORATORY · PI BOYDEN, EDWARD S., ZADOR, ANTHONY M · 2020 to 2020
$3.3M
Mechanisms of pathology and neuronal hyperactivity in a memory circuit in Alzheimer's diseaseR01AG070831 · NIA · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S., TSAI, LI-HUEI · 2021 to 2025
$3.2M
Multiplexed Nanoscale Protein Mapping Through Expansion Microscopy and Immuno-SABERRF1MH124606 · NIMH · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S., YIN, PENG · 2020 to 2020
$2.7M
Construction of an integrated immune - vascular brain - chip as a platform for the study, drug screening, and treatments of Alzheimer's diseaseUG3NS115064 · NINDS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BLANCHARD, JOEL WILLIAM, KELLIS, MANOLIS · 2019 to 2020
$2.5M
The infectious etiology of Alzheimer's disease revealed at nanoscale precisionR56AG069192 · NIA · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI AN, BOBAE · 2020 to 2020
$1.5M
NIAID NIH HHS P30 AI060354NIA NIH HHS F32 AG072813NIA NIH HHS P30 AG010161NIA NIH HHS P30 AG072975NIA NIH HHS R01 AG015819NIA NIH HHS R01 AG017917NIA NIH HHS R01 AG058639NIA NIH HHS R01 AG070831NIA NIH HHS R56 AG069192NIBIB NIH HHS R01 EB024261NIMH NIH HHS RF1 MH123403NIMH NIH HHS RF1 MH124606NINDS NIH HHS UG3 NS115064
6 · The paper itself

Abstract

Expansion revealing (ExR) elucidates cellular organization by separating proteins within dense nanostructures by 20x linear expansion, but requires fixation procedures incompatible with human pathology specimens. Here, we report ExR of pathology (ExRPath), which attains ~20 nm resolution and decrowding of such tissues, through iterative 20x expansion, adapted to human brain pathology specimens. We also report a single-shot 15x expansion protocol for such tissues (15ExMPath), achieved through one-shot 15x expansion. Applying ExRPath and 15ExMPath to COVID-19-decedent brain tissue reveals periodic amyloid nanoclusters that co-localize with SARS-CoV-2 in a rare minority of patient specimens, pointing to a potential neuroinflammatory phenotype associated with COVID-19, and highlighting the power of high-throughput nanoimaging, empowered by expansion microscopy, for discovering potential novel disease mechanisms.

Identifiers

PMID42182160
PMCPMC13192606

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.