Evidence map›Paper›PMID 42182072›Full record

ArticleJournal of orthopaedic translation2026

Type E endothelial cells orchestrate embryonic skeletogenesis via chondrogenic priming of skeletal progenitors.

Yang-Yang Li, Sha Huang, Ou Hu, Qin Qin, Pulin Yan, Peng Lin, Ying-Bo Wang, Huai-Jian Jin, Jian Wu, Yu-Tong Wu and 8 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of orthopaedic translation, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Yang-Yang LiDepartment of Spine Surgery, Center of Orthopedics, State Key Laboratory of Trauma and Chemical Poisoning, Chongqing Key Laboratory of Spinal Disease Therapy and Regeneration (Military-Civilian), Daping Hospital, Army Medical University, Chongqing, 400042, China.
Sha HuangDepartment of Spine Surgery, Center of Orthopedics, State Key Laboratory of Trauma and Chemical Poisoning, Chongqing Key Laboratory of Spinal Disease Therapy and Regeneration (Military-Civilian), Daping Hospital, Army Medical University, Chongqing, 400042, China.
Ou HuDepartment of Spine Surgery, Center of Orthopedics, State Key Laboratory of Trauma and Chemical Poisoning, Chongqing Key Laboratory of Spinal Disease Therapy and Regeneration (Military-Civilian), Daping Hospital, Army Medical University, Chongqing, 400042, China.
Qin QinDepartment of Spine Surgery, Center of Orthopedics, State Key Laboratory of Trauma and Chemical Poisoning, Chongqing Key Laboratory of Spinal Disease Therapy and Regeneration (Military-Civilian), Daping Hospital, Army Medical University, Chongqing, 400042, China.
Pulin YanDepartment of Spine Surgery, Center of Orthopedics, State Key Laboratory of Trauma and Chemical Poisoning, Chongqing Key Laboratory of Spinal Disease Therapy and Regeneration (Military-Civilian), Daping Hospital, Army Medical University, Chongqing, 400042, China.
Peng LinDepartment of Spine Surgery, Center of Orthopedics, State Key Laboratory of Trauma and Chemical Poisoning, Chongqing Key Laboratory of Spinal Disease Therapy and Regeneration (Military-Civilian), Daping Hospital, Army Medical University, Chongqing, 400042, China.
Ying-Bo WangDepartment of Spine Surgery, Center of Orthopedics, State Key Laboratory of Trauma and Chemical Poisoning, Chongqing Key Laboratory of Spinal Disease Therapy and Regeneration (Military-Civilian), Daping Hospital, Army Medical University, Chongqing, 400042, China.
Huai-Jian JinDepartment of Spine Surgery, Center of Orthopedics, State Key Laboratory of Trauma and Chemical Poisoning, Chongqing Key Laboratory of Spinal Disease Therapy and Regeneration (Military-Civilian), Daping Hospital, Army Medical University, Chongqing, 400042, China.
Jian WuDepartment of Spine Surgery, Center of Orthopedics, State Key Laboratory of Trauma and Chemical Poisoning, Chongqing Key Laboratory of Spinal Disease Therapy and Regeneration (Military-Civilian), Daping Hospital, Army Medical University, Chongqing, 400042, China.
Yu-Tong WuDepartment of Spine Surgery, Center of Orthopedics, State Key Laboratory of Trauma and Chemical Poisoning, Chongqing Key Laboratory of Spinal Disease Therapy and Regeneration (Military-Civilian), Daping Hospital, Army Medical University, Chongqing, 400042, China.
Na LuoDepartment of Spine Surgery, Center of Orthopedics, State Key Laboratory of Trauma and Chemical Poisoning, Chongqing Key Laboratory of Spinal Disease Therapy and Regeneration (Military-Civilian), Daping Hospital, Army Medical University, Chongqing, 400042, China.
Qing-Hua MaInstitute of Pathology and Southwest Cancer Center, Southwest Hospital, Third Military Medical University (Amy Medical University), The Key Laboratory of Tumor Immunopathology, The Ministry of Education of China, Chongqing, 400038, China.
Rui-Li CaiInstitute of Pathology and Southwest Cancer Center, Southwest Hospital, Third Military Medical University (Amy Medical University), The Key Laboratory of Tumor Immunopathology, The Ministry of Education of China, Chongqing, 400038, China.
Yang-Li XieCenter of Bone Metabolism and Repair, State Key Laboratory of Trauma and Chemical Poisoning, Trauma Center, Research Institute of Surgery, Laboratory for the Prevention and Rehabilitation of Military Training Related Injuries, Army Medical Center of PLA (Daping Hospital), Army Medical University, Chongqing, 400042, China.
Lin ChenCenter of Bone Metabolism and Repair, State Key Laboratory of Trauma and Chemical Poisoning, Trauma Center, Research Institute of Surgery, Laboratory for the Prevention and Rehabilitation of Military Training Related Injuries, Army Medical Center of PLA (Daping Hospital), Army Medical University, Chongqing, 400042, China.
Yi-Bo GanDepartment of Spine Surgery, Center of Orthopedics, State Key Laboratory of Trauma and Chemical Poisoning, Chongqing Key Laboratory of Spinal Disease Therapy and Regeneration (Military-Civilian), Daping Hospital, Army Medical University, Chongqing, 400042, China.
Peng LiuDepartment of Spine Surgery, Center of Orthopedics, State Key Laboratory of Trauma and Chemical Poisoning, Chongqing Key Laboratory of Spinal Disease Therapy and Regeneration (Military-Civilian), Daping Hospital, Army Medical University, Chongqing, 400042, China.
Jian HeDepartment of Spine Surgery, Center of Orthopedics, State Key Laboratory of Trauma and Chemical Poisoning, Chongqing Key Laboratory of Spinal Disease Therapy and Regeneration (Military-Civilian), Daping Hospital, Army Medical University, Chongqing, 400042, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Skeletal stem/progenitor cells (SSPCs) are pivotal orchestrators of embryonic skeletogenesis, underlying chondrogenesis and osteogenesis, however, the molecular determinants governing SSPC functionality remain elusive. Methods: We performed single-cell RNA sequencing (scRNA-seq) to delineate cellular heterogeneity within the developing limb bud and to identify candidate cellular markers. The spatiotemporal distribution of target cell populations was further examined via immunofluorescence staining. Fluorescence-activated cell sorting (FACS) was used to isolate endothelial cells and SSPCs based on surface markers. Functional properties of these cells were assessed using in vitro assays, and direct co-culture experiments were conducted to evaluate intercellular communication and underlying molecular pathways. Results: We delineate a CD140a Conclusion: These findings uncover a fundamental mechanism of long bone development mediated by CD140a The translational potential of this article: By elucidating the regulatory function of type E endothelial cells in orchestrating chondrogenic priming during embryonic skeletogenesis, this study unveils potential therapeutic strategies for bone regeneration through targeting the vascular network.

Indexed as

BMP signalingEmbryonic developmentEndochondral ossificationEndothelial cellLimb budSkeletal stem/progenitor cell

Identifiers

PMID42182072
PMCPMC13197703

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.