Evidence map›Paper›PMID 42181281›Full record

ArticleiScience2026

JACUZI-SD: An automated, high-throughput, minimally stressful approach to sleep depriving larval zebrafish.

Leah J Elias, Harrison Khoo, François Kroll, Caroline Zhang, Soojung C Hur, Jason Rihel, Seth Blackshaw

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors.

Leah J EliasDepartment of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Harrison KhooDepartment of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, USA.
François KrollSorbonne Université, INSERM U968, CNRS UMR 7210, Institut de la Vision, Paris, France.
Caroline ZhangDepartment of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Soojung C HurDepartment of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, USA.
Jason RihelDepartment of Cell and Developmental Biology, University College London, London, UK.
Seth BlackshawDepartment of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Inadequate sleep is common and broadly disrupts well-being. A fundamental challenge to modeling sleep deprivation (SD) experimentally is the introduction of confounds such as stress or circadian manipulation. We therefore developed JACUZI-SD (jetting automated currents under zebrafish to induce sleep deprivation), an automated, high-throughput, and minimally stressful system to SD larval zebrafish during the dark cycle. JACUZI-SD delivers randomized water pulses via a custom milli-fluidic device integrated with a 96-well plate, reducing total sleep time by 41%-64% and eliciting a rebound sleep characterized by an 11% increase in total sleep time and 29% increase in sleep bout length. Water pulses are minimally stressful, eliciting less

Indexed as

biological sciences

Identifiers

PMID42181281
PMCPMC13196093

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.