Live-cell imaging of endogenous Egr1 mRNA using an MPBS knock-in mouse model.

Hyerim Ahn, Hyeonjeong Jeong, Dong Wook Kim, Jae Youn Shim, Hye Yoon Park

Abstract read

In one paragraph

Article in iScience, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Hyerim AhnDepartment of Electrical and Computer Engineering, College of Science and Engineering, University of Minnesota, Minneapolis, MN 55455, USA.

Hyeonjeong JeongDepartment of Electrical and Computer Engineering, College of Science and Engineering, University of Minnesota, Minneapolis, MN 55455, USA.

Dong Wook KimDepartment of Electrical and Computer Engineering, College of Science and Engineering, University of Minnesota, Minneapolis, MN 55455, USA.

Jae Youn ShimDepartment of Electrical and Computer Engineering, College of Science and Engineering, University of Minnesota, Minneapolis, MN 55455, USA.

Hye Yoon ParkDepartment of Electrical and Computer Engineering, College of Science and Engineering, University of Minnesota, Minneapolis, MN 55455, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Real-time imaging of endogenous mRNA is essential for understanding the dynamics of gene regulation, yet appropriate animal models have been limited. We developed an Egr1-MPBS knock-in mouse line in which all endogenous Egr1 transcripts are tagged with 12 tandem MS2 and PP7 binding site (MPBS) pairs, enabling direct visualization of single mRNA molecules under physiological conditions. To establish proof of principle, we derived mouse embryonic fibroblast (MEF) lines from this model and performed live-cell imaging of fluorescently labeled Egr1 transcripts. We found that the insertion of the MPBS cassette did not alter endogenous mRNA stability or expression dynamics. This system allowed us to observe Egr1 transcriptional bursting and quantitatively track individual mRNA molecules as they diffused within the nucleus and cytoplasm. By integrating technological innovation with mechanistic insight, the Egr1-MPBS mouse establishes a broadly applicable platform for quantitative studies of gene expression and RNA trafficking at single-molecule resolution in living systems.

Indexed as

molecular biologymolecular mechanism of gene regulation

Identifiers

PMID42181261

DOI10.1016/j.isci.2026.115955

PMCPMC13197786

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.