ArticleMultiple sclerosis journal - experimental, translational and clinical
Validation of an alternative algorithm to oligoclonal bands. Free Light Chains in Cerebrospinal Fluid: Quantitative and Qualitative method.
Article in Multiple sclerosis journal - experimental, translational and clinical. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: Nearly 85% of patients with Multiple Sclerosis (MS) present oligoclonal bands (OB) by isoelectric focusing (IEF) in their cerebrospinal fluid (CSF). Currently, it is proposed to quantify free light chains (FLC), as a potential alternative for the analysis of intrathecal synthesis of immunoglobulins (ISI). Objectives: To study FLC in CSF/serum by qualitative/quantitative methods, in comparison with the IEF. Methods: Paired CSF/serum samples from 81 patients with a medical request for OB by IEF were analyzed. The FLC index was calculated: CSF/serum FLC quotient with reference to the albumin CSF/serum concentration (κ-Index and λ-Index). To analyze the dimer-monomer patterns of FLC in CSF-serum, qualitative Western blot (WB) immunoassay was performed with anti-κ and anti-λ antisera. Results: A cut-off limit was obtained for κ-Index=20 and λ-Index=17, sensitivity 70% and 28%; and specificity 95% and 92%, respectively. The WB was positive when a higher proportion of κ-monomer (WBκ) and/or λ-dimer (WBλ) was observed in CSF versus serum. Sensitivity for WBκ and WBλ was 80% and 82% respectively, specificity of 93% for both. The combined use of κ-Index<20 and the presence of κ-monomer and/or λ-dimer by WB increased the sensitivity to 100%. Conclusions: We propose analysis by WB when κ-Index<20, as an alternative algorithm to OB by IEF, for the investigation of ISI in CSF.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.