ArticleBurns & trauma2026
Negative pressure-induced fibroblast activation for wound healing through the Piezo1-Ca
Article in Burns & trauma, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Background: Negative pressure wound therapy (NPWT) is widely used to promote wound healing, yet the mechanotransduction mechanisms underlying its efficacy remain unclear because of the absence of precise and controllable Methods: We developed a high-precision negative pressure loading device compatible with six-well plates, facilitating independent and intelligent control over negative pressure values, durations, and modes for each well. To validate the uniformity and stability of the negative pressure environment, finite element analysis (FEA) was implemented. The mechanism was explored using proteomic profiling of negative pressure-treated fibroblasts, complemented by molecular interrogation of Piezo1 expression and calcium signaling dynamics. The results of functional studies integrated genetic silencing and pharmacological modulation of the pathway, with Results: FEA confirmed a stable pressure distribution within the negative pressure chamber. The cells experienced predominantly compressive stresses that scaled linearly with the applied pressure, reaching -2 kPa at -40 mmHg. We found that applying a continuous negative pressure of -40 mmHg for 2 hours significantly activated dermal fibroblasts. TMT-based proteomic analysis revealed the upregulation of Piezo1 in negative pressure-treated dermal fibroblasts, which was further confirmed by molecular and immunohistochemical analyses of both cellular and granulation tissue samples. Pharmacological inhibition or knockdown of Piezo1 attenuated negative pressure-induced dermal fibroblast activation Conclusions: This study revealed that the Piezo1-Ca
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