Evidence map›Paper›PMID 42180354›Full record

ArticlemedRxiv : the preprint server for health sciences2026

Liquid Biopsy of HPV Cell-Free DNA Enables Blood-Based Early Detection and Molecular Stratification of HPV-Associated Cancer and Precancer Stages.

Qin Wang, Samuli Eldfors, Sangmi Sandra Lee, Dipon Das, Yana Al-Inaya, Gjystina Lumaj, Eliana T Epstein, Shriya Shukla, Emma Ricart, Harsharan Dhillon and 7 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in medRxiv : the preprint server for health sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Qin WangDepartment of Otolaryngology-Head and Neck Surgery, Harvard Medical School, Boston, MA, USA.
Samuli EldforsDepartment of Otolaryngology-Head and Neck Surgery, Harvard Medical School, Boston, MA, USA.ORCID 0000-0001-5056-4750
Sangmi Sandra LeeBroad Institute of MIT and Harvard, Cambridge, MA, USA.
Dipon DasDepartment of Otolaryngology-Head and Neck Surgery, Harvard Medical School, Boston, MA, USA.
Yana Al-InayaDepartment of Otolaryngology-Head and Neck Surgery, Harvard Medical School, Boston, MA, USA.
Gjystina LumajDepartment of Otolaryngology-Head and Neck Surgery, Harvard Medical School, Boston, MA, USA.
Eliana T EpsteinMassachusetts General Hospital, Boston, MA, USA.
Shriya ShuklaDepartment of Otolaryngology-Head and Neck Surgery, Harvard Medical School, Boston, MA, USA.
Emma RicartBroad Institute of MIT and Harvard, Cambridge, MA, USA.
Harsharan DhillonPacific Biosciences, Menlo Park, CA, USA.
Juniper LakePacific Biosciences, Menlo Park, CA, USA.
Shun HirayamaDepartment of Otolaryngology-Head and Neck Surgery, Harvard Medical School, Boston, MA, USA.
Viktor A AdalsteinssonBroad Institute of MIT and Harvard, Cambridge, MA, USA.
Michael G DrageDepartment of Pathology, Mass General Brigham, Harvard Medical School, Boston, MA, USA.
Doga C GulhanBroad Institute of MIT and Harvard, Cambridge, MA, USA.
Benjamin T DavisMassachusetts General Hospital, Boston, MA, USA.
Daniel L FadenDepartment of Otolaryngology-Head and Neck Surgery, Harvard Medical School, Boston, MA, USA.

Funding

The impact of HIV viral diversity and cellular immunity on HIV pathogenesisP30AI060354 · NIAID · HARVARD UNIVERSITY (MEDICAL SCHOOL) · PI Daniel Lingwood · 2004 to 2026
$94.4M
NIAID NIH HHS P30 AI060354
6 · The paper itself

Abstract

Liquid biopsies targeting circulating tumor DNA enable noninvasive cancer detection but lack sensitivity in pre- and early- cancer stages, where clinical benefits would be greatest. Human papillomavirus (HPV) causes six cancer types, accounting for 5% of all cancers worldwide. Targeting HPV cell-free (cf)DNA offers a compelling opportunity to overcome current liquid biopsy constraints due to its unique tumor-specific origin, lack of sequence homology to the human genome, and the high viral-to-human copy ratio per cell. Utilizing HPV-associated anal cancer and precancer as a model, here we applied a custom, multi-feature HPV whole-genome liquid biopsy to biobanked and prospective screening cohorts spanning the HPV infection-precancer-cancer continuum. HPV cfDNA was detected years before cancer diagnosis and as early as the infection stage, with increasing detection as stages advanced. Genomic hallmarks of HPV malignancy, including HPV integration,

Identifiers

PMID42180354
PMCPMC13193089

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.