ArticleJournal of advanced veterinary and animal research2026
Molecular identification of ligninolytic fungi from West Sumatra, Indonesia, using the Internal Transcribed Spacer (ITS) region.
Article in Journal of advanced veterinary and animal research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Objectives: This study aimed to isolate and identify ligninolytic fungi from decayed wood in West Sumatra, Indonesia, with a focus on their potential applications in animal production technology. The goal was to explore the potential of these fungi to improve lignocellulosic agricultural waste degradation, thereby enhancing the digestibility and nutritional value of ruminant feed. Materials and Methods: Fungi were isolated from rotting wood samples collected in Padang and Solok. The Bavendamm test was used to screen for ligninolytic activity. DNA was extracted from purified isolates, and PCR amplification of the ITS region was performed using ITS1 and ITS4 primers. Amplified products were sequenced and analyzed using BLAST against the NCBI GenBank database for species identification. Results: Two of four purified fungal isolates (P1 and S2) showed positive results in the Bavendamm test, indicating their ligninolytic potential. PCR amplification produced 574 bp (P1) fragments and 586 bp (S2). BLAST analysis identified P1 as Conclusions: Identifying
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