Evidence map›Paper›PMID 42180257›Full record

ArticleFrontiers in cellular and infection microbiology2026

Rapid and simultaneous detection of

Qinglin Ma, Zhihua He, Guikai Duan, Jiahao Wei, Chun Duan, Yan Zhu, Feng Long

Abstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in cellular and infection microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Qinglin MaDepartment of Clinical Laboratory, Shenzhen Maternity and Child Healthcare Hospital, Southern Medical University, Shenzhen, Guangdong, China.
Zhihua HeDepartment of Clinical Laboratory, Shenzhen Maternity and Child Healthcare Hospital, Southern Medical University, Shenzhen, Guangdong, China.
Guikai DuanDepartment of Clinical Laboratory, Shenzhen Maternity and Child Healthcare Hospital, Southern Medical University, Shenzhen, Guangdong, China.
Jiahao WeiShenzhen School of Clinical Medicine, Southern Medical University, Shenzhen, Guangdong, China.
Chun DuanDepartment of Clinical Laboratory, Shenzhen Maternity and Child Healthcare Hospital, Southern Medical University, Shenzhen, Guangdong, China.
Yan ZhuDepartment of Clinical Laboratory, Shenzhen Maternity and Child Healthcare Hospital, Southern Medical University, Shenzhen, Guangdong, China.
Feng LongDepartment of Clinical Laboratory, Shenzhen Maternity and Child Healthcare Hospital, Southern Medical University, Shenzhen, Guangdong, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Invasive fungal infections (IFIs) caused by Methods: We developed and optimized a duplex real-time recombinase polymerase amplification (RPA) assay targeting the internal transcribed spacer (ITS) regions of both fungi. Analytical performance, including specificity, sensitivity, limit of detection (LOD), repeatability and reproducibility, was systematically evaluated. The assay's diagnostic applicability was assessed using 26 clinical specimens and compared with culture and a duplex qPCR assay. Results: The assay demonstrated high specificity with no cross-reactivity against non-target fungi or human DNA. The LODs were 559.2 fg/reaction (~34.7 copies) for Conclusion: This rapid, sensitive, and specific duplex real-time RPA assay represents a potential point-of-care testing (POCT) tool for the early and simultaneous diagnosis of two major IFIs-causing pathogens.

Indexed as

Candida albicansCryptococcosisCryptococcus neoformansInvasive Fungal InfectionsMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesPoint-of-Care SystemsReal-Time Polymerase Chain ReactionDNA, FungalHumansLimit of DetectionRapid Diagnostic TestsReproducibility of ResultsSensitivity and SpecificityDNA, FungalCandida albicansCryptococcus neoformansduplex real-time RPAinvasive fungal infectionsmolecular diagnosticspoint-of-care testing

Identifiers

PMID42180257
PMCPMC13189755

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.