Tandem Oligonucleotide Synthesis Enabled by a Reducible Linker.

Abby H Sham, Evan Zakaria, Nicholas G Horton, Derek K O'Flaherty

Abstract read

In one paragraph

Article in Chembiochem : a European journal of chemical biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Tandem Oligonucleotide Synthesis Enabled by a Reducible Linker.Chembiochem : a European journal of chemical biology · 2026
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Abby H ShamDepartment of Chemistry, University of Guelph, Guelph, Ontario, Canada.ORCID https://orcid.org/0009-0002-1241-8933

Evan ZakariaDepartment of Chemistry, University of Guelph, Guelph, Ontario, Canada.ORCID https://orcid.org/0009-0001-8983-7311

Nicholas G HortonDepartment of Chemistry, University of Guelph, Guelph, Ontario, Canada.ORCID https://orcid.org/0009-0006-6924-9551

Derek K O'FlahertyDepartment of Chemistry, University of Guelph, Guelph, Ontario, Canada.ORCID https://orcid.org/0000-0003-3693-6380

Funding

Canadian Foundation for Innovation - John R. Evans Leaders 43757 and 45118HFSP Research Grant-Early Career RGY0062/2022NSERC Alliance Catalyst ALLRP 57555822NSERC Discovery RGPIN 2020-05043

6 · The paper itself

Abstract

Tandem oligonucleotide synthesis (TOS) is accomplished via the introduction of a cleavable backbone linker within a single sequence. Multiple oligonucleotide fragments are released upon immolation of the cleavable linker(s). Here, we revisit the use of a commercially available disulfide-containing building block, typically utilized for the introduction of terminal thiol functionality in DNA/RNA oligomers to perform TOS in various contexts. As expected, the disulfide linkage is orthogonal to the standard DNA/RNA production workflow. Upon reduction, the disulfide is efficiently cleaved, resulting in the corresponding strand fragments, each containing thiol modification(s). We showcase the utility of this methodology by assembling a model crosslinked small interfering RNA (siRNA) construct. Furthermore, we report compatibility of this TOS with selected (bio)conjugation techniques. Overall, our development will find applications in various biotechnology contexts.

Indexed as

OligonucleotidesDisulfidesOxidation-ReductionRNA, Small InterferingSulfhydryl CompoundsDisulfidesOligonucleotidesRNA, Small InterferingSulfhydryl Compounds

Identifiers

PMID42179012

DOI10.1002/cbic.70364

PMCPMC13206200

What OpenQuestion holds

Textmetadata

LicenceCC BY-NC-ND

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.